Genetic control of leishmania infection in OcB-43 recombinant congenic strain

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F14%3A00300749" target="_blank" >RIV/68407700:21460/14:00300749 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genetic control of leishmania infection in OcB-43 recombinant congenic strain

Popis výsledku v původním jazyce

Leishmaniasis is a group of diseases caused by intracellular protozoan parasites of the genus Leishmania. Depending on the species of parasite and host genetic the disease can be localize in skin or can spread into internal organs and cause visceral pathology. Leishmaniasis is a complex disease which is controlled by many genes and network of gene-gene interactions. Leishmania major generally causes skin lesions in humans, in mice it can result both skin lesions and visceral pathology (hepatomegaly and splenomegaly). To dissect genetic control of leishmaniasis after L. major infection, we analysed 228 infected F2 hybrids between O20xOcB-43. The mice were infected with L. major by injecting 107 parasites and development of lesions was recorded every week. DNA samples were isolated by proteinase method and B10.O20-derived segments typed by PCR method. The statistical analysis showed that a segment on chromosome 2 (D2Mit37) carrying potential gene/loci which controls lesion development (P=0,0083, corrected P=0,14). Also statistical analysis revealed to identify suggestive loci in the same region which controls hepatomegaly (P=0,0074, corrected P=0,12).

Název v anglickém jazyce

Genetic control of leishmania infection in OcB-43 recombinant congenic strain

Popis výsledku anglicky

Leishmaniasis is a group of diseases caused by intracellular protozoan parasites of the genus Leishmania. Depending on the species of parasite and host genetic the disease can be localize in skin or can spread into internal organs and cause visceral pathology. Leishmaniasis is a complex disease which is controlled by many genes and network of gene-gene interactions. Leishmania major generally causes skin lesions in humans, in mice it can result both skin lesions and visceral pathology (hepatomegaly and splenomegaly). To dissect genetic control of leishmaniasis after L. major infection, we analysed 228 infected F2 hybrids between O20xOcB-43. The mice were infected with L. major by injecting 107 parasites and development of lesions was recorded every week. DNA samples were isolated by proteinase method and B10.O20-derived segments typed by PCR method. The statistical analysis showed that a segment on chromosome 2 (D2Mit37) carrying potential gene/loci which controls lesion development (P=0,0083, corrected P=0,14). Also statistical analysis revealed to identify suggestive loci in the same region which controls hepatomegaly (P=0,0074, corrected P=0,12).

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/EE.2.3.20.0092" target="_blank" >EE.2.3.20.0092: Rozvoj výzkumného týmu BIO-OPT-XUV na FBMI ČVUT</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů