Methods of the genetic engineering for mapping of genes that influence response to parasite Leishmania major

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Methods of the genetic engineering for mapping of genes that influence response to parasite Leishmania major

Popis výsledku v původním jazyce

Leishmaniasis is a parasitic disease caused by protozoan of genus Leishmania. These protozoans are transmitted to human body by sandflies (Phlebotomus, Lutzomyia, Psychodopygus) and cause three main forms of pathology: cutaneous, mucocutaneous and visceral. Leishmaniasis is one of the most widespreaddiseases, although is also one of the most neglected diseases in the world, that’s why this issue has different support from WHO. Different species of Leishmania induce duration length and types of manifestation, but even the patients infected by the same species develop different clinical manifestations. Mouse model is frequently used for examination of genetic influence on disease susceptibility, because mouse genome is in 92 % homologous to the human genome. Advance in molecular biology methods, especially genotyping, gave us a tool to study the role of host genotype in a control of several immunological and pathological parameters of leishmaniasis: skin lesion size, splenomegaly, hepatomegaly and parasiteload in organs. We have prepared F2 hybrids between strain OcB-42 and OcB-43, which differ in chromosomal segments on chromosomes 3, 9 and 15. Mice were infected by L. major for 8 weeks and their phenotypes were established. We are currently DNA typing segments on chromosomes 3, 9 and 15. Influence of these segments on tested parameters will be statistically evaluated by ANOVA using Statistica software.

Název v anglickém jazyce

Methods of the genetic engineering for mapping of genes that influence response to parasite Leishmania major

Popis výsledku anglicky

Leishmaniasis is a parasitic disease caused by protozoan of genus Leishmania. These protozoans are transmitted to human body by sandflies (Phlebotomus, Lutzomyia, Psychodopygus) and cause three main forms of pathology: cutaneous, mucocutaneous and visceral. Leishmaniasis is one of the most widespreaddiseases, although is also one of the most neglected diseases in the world, that’s why this issue has different support from WHO. Different species of Leishmania induce duration length and types of manifestation, but even the patients infected by the same species develop different clinical manifestations. Mouse model is frequently used for examination of genetic influence on disease susceptibility, because mouse genome is in 92 % homologous to the human genome. Advance in molecular biology methods, especially genotyping, gave us a tool to study the role of host genotype in a control of several immunological and pathological parameters of leishmaniasis: skin lesion size, splenomegaly, hepatomegaly and parasiteload in organs. We have prepared F2 hybrids between strain OcB-42 and OcB-43, which differ in chromosomal segments on chromosomes 3, 9 and 15. Mice were infected by L. major for 8 weeks and their phenotypes were established. We are currently DNA typing segments on chromosomes 3, 9 and 15. Influence of these segments on tested parameters will be statistically evaluated by ANOVA using Statistica software.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10610 - Biophysics

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů