Methods of the genetic engineering for analysis of gene expression in noninfected mice and mice infected with parasite Leishmania major

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Methods of the genetic engineering for analysis of gene expression in noninfected mice and mice infected with parasite Leishmania major

Popis výsledku v původním jazyce

Leishmaniasis is one of the most neglected diseases. It is caused by protozoan parasite of genus Leishmania. The disease is spread by bite of Phlebotomusor Lutzomia sandfly. Leishmaniasis is a health problem in 98 countries worldwide. There are more than million new cases and about 50000 deaths each year. That is why there is a support from WHO. We present use methodsof molecular biology for examination of influence of genetic segments on chromosomes 3, 9 and 15 on phenotypicmanifestions of L. major infection in F2 generation derived from strains OcB-42 and OcB-43, which differ in these segments. We focused on genotyping method, statistical evaluation using Statistica and gene expression analysis for selecting the candidate genes of interest. Definition of global gene expression is monitored using DNA microarrays, with the validation of results by real-time PCR. We aim to identify genes and functions controlling this disease. We monitored several imunopatologicalparameters such as lesion size, splenomegaly, hepatomegaly, parasite load in organs. All these parameters were analysed 8 weeks after infection with L. major.

Název v anglickém jazyce

Methods of the genetic engineering for analysis of gene expression in noninfected mice and mice infected with parasite Leishmania major

Popis výsledku anglicky

Leishmaniasis is one of the most neglected diseases. It is caused by protozoan parasite of genus Leishmania. The disease is spread by bite of Phlebotomusor Lutzomia sandfly. Leishmaniasis is a health problem in 98 countries worldwide. There are more than million new cases and about 50000 deaths each year. That is why there is a support from WHO. We present use methodsof molecular biology for examination of influence of genetic segments on chromosomes 3, 9 and 15 on phenotypicmanifestions of L. major infection in F2 generation derived from strains OcB-42 and OcB-43, which differ in these segments. We focused on genotyping method, statistical evaluation using Statistica and gene expression analysis for selecting the candidate genes of interest. Definition of global gene expression is monitored using DNA microarrays, with the validation of results by real-time PCR. We aim to identify genes and functions controlling this disease. We monitored several imunopatologicalparameters such as lesion size, splenomegaly, hepatomegaly, parasite load in organs. All these parameters were analysed 8 weeks after infection with L. major.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10610 - Biophysics

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů