Analyses of emission spectra of biological fluorophores

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F17%3A00330878" target="_blank" >RIV/68407700:21460/17:00330878 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analyses of emission spectra of biological fluorophores

Popis výsledku v původním jazyce

Fluorophores are chemical compounds that can re-emit light upon light excitation. In our organism, we can find many types of bio-fluorophores. The most interestingis NADH that plays an important role in the respiratory chain embedded in the mitochondria. Much pathology included cancer and others diseases in human body is related to dysfunction of mitochondria and variation of ATP production.Our motivation is to use NADH as a biomarker for cell’s viability. By preparing NADH in media of various pH,we would determine the differences in their fluorescence emission spectra recorded using three excitation wavelengths –340nm, 350 and 365nm. The fluorescence spectrum of the mixture of NADH with other two bio-fluorophores (pyridoxine and tryptophan) was analyzed by deconvoluting SW GASPED, to recognize the contribution of NADH in this spectrum. The all measurementswere performed on a Shimadzu UV-3600 spectrophotometer and a Fluoromax-4 fluorimeter. The important parameter of fluorescence, quantum yield, was also calculated. The experiment and the analyses of fluorescence spectra were also applied to the yeast.

Název v anglickém jazyce

Analyses of emission spectra of biological fluorophores

Popis výsledku anglicky

Fluorophores are chemical compounds that can re-emit light upon light excitation. In our organism, we can find many types of bio-fluorophores. The most interestingis NADH that plays an important role in the respiratory chain embedded in the mitochondria. Much pathology included cancer and others diseases in human body is related to dysfunction of mitochondria and variation of ATP production.Our motivation is to use NADH as a biomarker for cell’s viability. By preparing NADH in media of various pH,we would determine the differences in their fluorescence emission spectra recorded using three excitation wavelengths –340nm, 350 and 365nm. The fluorescence spectrum of the mixture of NADH with other two bio-fluorophores (pyridoxine and tryptophan) was analyzed by deconvoluting SW GASPED, to recognize the contribution of NADH in this spectrum. The all measurementswere performed on a Shimadzu UV-3600 spectrophotometer and a Fluoromax-4 fluorimeter. The important parameter of fluorescence, quantum yield, was also calculated. The experiment and the analyses of fluorescence spectra were also applied to the yeast.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů