Comparison of fluorescence spectra of healthy and carcinoma cell cultures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F18%3A00326382" target="_blank" >RIV/68407700:21460/18:00326382 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of fluorescence spectra of healthy and carcinoma cell cultures

Popis výsledku v původním jazyce

NADH emission spectra analysis is a promising method of photomedicine to determine the vitality of cells and to obtain information about the metabolic processes in them. The aim of this work is to find the differences in emission spectra between cell cultures of healthy and tumor cells by analyzing the influence of individual contributions from the biofluorophores contained in the cell at the excitation of 260 nm, 340 nm, and 365nm. 260 nm and 340 nm are the excitation maxima of NADH. 365 nm is the excitation wavelength we had found that gives the most intense fluorescence of NADH compares to the rest of biofluorophores. The influence of different cultivation media, glucose adding and oxygen reduction of cell samples on the fluorescence spectra are shown. The experiments were made with the cell cultures of cell line CT26 and 3T3. Measured data were treated by software GASpeD (Genetic Algorithm Spectra Decomposition).

Název v anglickém jazyce

Comparison of fluorescence spectra of healthy and carcinoma cell cultures

Popis výsledku anglicky

NADH emission spectra analysis is a promising method of photomedicine to determine the vitality of cells and to obtain information about the metabolic processes in them. The aim of this work is to find the differences in emission spectra between cell cultures of healthy and tumor cells by analyzing the influence of individual contributions from the biofluorophores contained in the cell at the excitation of 260 nm, 340 nm, and 365nm. 260 nm and 340 nm are the excitation maxima of NADH. 365 nm is the excitation wavelength we had found that gives the most intense fluorescence of NADH compares to the rest of biofluorophores. The influence of different cultivation media, glucose adding and oxygen reduction of cell samples on the fluorescence spectra are shown. The experiments were made with the cell cultures of cell line CT26 and 3T3. Measured data were treated by software GASpeD (Genetic Algorithm Spectra Decomposition).

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů