Influence of pH environment on fluorescence spectra of samples of healthy and carcinoma cell cultures

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Influence of pH environment on fluorescence spectra of samples of healthy and carcinoma cell cultures

Popis výsledku v původním jazyce

Influence of pH environment on fluorescence spectra (FS) of NADH is studied on the base of changes these spectra for sample environment pH 5, 7 and 8 and temperature 20 °C. The samples are prepared as suspensions of healthy and carcinoma cell cultures line BALB/3T3 and CT26B in PBS buffer with pH 5, 7, 8 and temperature 20 °C. The normalized FS for excitation wavelength 365 nm were compared for healthy and tumor cells and cannot distinguish between position of the maximum intensity for healthy and tumor cells. The GASpeD program was used to decompose the normalized FS for more detail its analyses. The optimal number of spectra decomposition curves was set at 4. It was found a difference in the concentration of free and bound NADH, with more free than bound NADH in healthy cells, in tumor cells it is the opposite. By comparing normalized FS for the same cells and pH 7, it is assumed that it does not depend on cell concentration at temperature 20 °C. The effect of pH on FS of healthy and tumor cells is the change in concentration of NADH in healthy cells and in concentration of FAD in tumor cells.

Název v anglickém jazyce

Influence of pH environment on fluorescence spectra of samples of healthy and carcinoma cell cultures

Popis výsledku anglicky

Influence of pH environment on fluorescence spectra (FS) of NADH is studied on the base of changes these spectra for sample environment pH 5, 7 and 8 and temperature 20 °C. The samples are prepared as suspensions of healthy and carcinoma cell cultures line BALB/3T3 and CT26B in PBS buffer with pH 5, 7, 8 and temperature 20 °C. The normalized FS for excitation wavelength 365 nm were compared for healthy and tumor cells and cannot distinguish between position of the maximum intensity for healthy and tumor cells. The GASpeD program was used to decompose the normalized FS for more detail its analyses. The optimal number of spectra decomposition curves was set at 4. It was found a difference in the concentration of free and bound NADH, with more free than bound NADH in healthy cells, in tumor cells it is the opposite. By comparing normalized FS for the same cells and pH 7, it is assumed that it does not depend on cell concentration at temperature 20 °C. The effect of pH on FS of healthy and tumor cells is the change in concentration of NADH in healthy cells and in concentration of FAD in tumor cells.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10610 - Biophysics

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Instruments and Methods for Biology and Medicine 2019

ISBN

978-80-01-06643-0

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

23-27

Název nakladatele

Česká technika - nakladatelství ČVUT, ČVUT v Praze

Místo vydání

Praha

Místo konání akce

Kladno

Datum konání akce

23. 5. 2019

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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