Analysis of fluorescence spectra of healthy cells as a function of carcinoma cell concentration, pH and temperature of environment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F21%3A00353305" target="_blank" >RIV/68407700:21460/21:00353305 - isvavai.cz</a>

Výsledek na webu

<a href="http://imbm.fbmi.cvut.cz/soubory/Sborniky/IMBM%202021" target="_blank" >http://imbm.fbmi.cvut.cz/soubory/Sborniky/IMBM%202021</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of fluorescence spectra of healthy cells as a function of carcinoma cell concentration, pH and temperature of environment

Popis výsledku v původním jazyce

Cancer diseases belong among the most common causes of death around the world. For their effective treatment an early detection of the disease is necessary. This study focuses on the option of detecting the cancerous cells by comparing the fluorescence spectra of bound and free NADH in healthy cells 3T3, cancerous cells CT26 and cancerous cells SNU475, and in a mixture of 3T3 and CT26 cells of different concentrations. Fluorescence spectra are measured at laboratory temperature and at 37 °C in pH of 7.2 and 6.2 with excitation of 365. Fluorescence spectra are analyzed with software GASpeD, which is based on genetic algorithm. This software breaks down the spectra into contributions of specific biofluorophores of the cells. The point of this study is to prove the possibility of distinguishing cancerous and healthy cells on the basis of their fluorescence spectra. The results show that there is a possibility to distinguish healthy and cancerous cells based on broken down spectra of their fluorescence for both pure cells and for their mixtures and at different temperatures and pH. This paper is based on a master´s thesis.

Název v anglickém jazyce

Analysis of fluorescence spectra of healthy cells as a function of carcinoma cell concentration, pH and temperature of environment

Popis výsledku anglicky

Cancer diseases belong among the most common causes of death around the world. For their effective treatment an early detection of the disease is necessary. This study focuses on the option of detecting the cancerous cells by comparing the fluorescence spectra of bound and free NADH in healthy cells 3T3, cancerous cells CT26 and cancerous cells SNU475, and in a mixture of 3T3 and CT26 cells of different concentrations. Fluorescence spectra are measured at laboratory temperature and at 37 °C in pH of 7.2 and 6.2 with excitation of 365. Fluorescence spectra are analyzed with software GASpeD, which is based on genetic algorithm. This software breaks down the spectra into contributions of specific biofluorophores of the cells. The point of this study is to prove the possibility of distinguishing cancerous and healthy cells on the basis of their fluorescence spectra. The results show that there is a possibility to distinguish healthy and cancerous cells based on broken down spectra of their fluorescence for both pure cells and for their mixtures and at different temperatures and pH. This paper is based on a master´s thesis.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů