Pneumatic Driven Pulsatile Perfusion Bioreactor for Tissue Enginered Vascular Replacements

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F24%3A00371295" target="_blank" >RIV/68407700:21460/24:00371295 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-3-031-62520-6_43" target="_blank" >http://dx.doi.org/10.1007/978-3-031-62520-6_43</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-031-62520-6_43" target="_blank" >10.1007/978-3-031-62520-6_43</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Pneumatic Driven Pulsatile Perfusion Bioreactor for Tissue Enginered Vascular Replacements

Popis výsledku v původním jazyce

Shear stress simulated in the bioreactor has a positive effect on the modulation of endothelial cells, their grow and gene expression. The aim of this work is to design and characterize a pressure wave-controlled perfusion system for stimulating both planar and tubular tissue in the culture chamber. This system must be able to deliver both small and large flow rates. Designed circuit consists of two large volume cartridges that alternately filled with culture medium. The flow direction is controlled by 3-way valves which are controlled by servo motors. The flow reading is based on the differential intensity of the LEDs through the filled and empty syringe. Servo motor control and signal processing from the photodiodes was realized on the Arduino platform. The assembled circuit was tested in a laboratory environment and subsequently used in a culture experiment with immortalized cells of the mouse endothelial line MS-1.

Název v anglickém jazyce

Pneumatic Driven Pulsatile Perfusion Bioreactor for Tissue Enginered Vascular Replacements

Popis výsledku anglicky

Shear stress simulated in the bioreactor has a positive effect on the modulation of endothelial cells, their grow and gene expression. The aim of this work is to design and characterize a pressure wave-controlled perfusion system for stimulating both planar and tubular tissue in the culture chamber. This system must be able to deliver both small and large flow rates. Designed circuit consists of two large volume cartridges that alternately filled with culture medium. The flow direction is controlled by 3-way valves which are controlled by servo motors. The flow reading is based on the differential intensity of the LEDs through the filled and empty syringe. Servo motor control and signal processing from the photodiodes was realized on the Arduino platform. The assembled circuit was tested in a laboratory environment and subsequently used in a culture experiment with immortalized cells of the mouse endothelial line MS-1.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

30201 - Cardiac and Cardiovascular systems

Projekt

<a href="/cs/project/NV19-02-00068" target="_blank" >NV19-02-00068: Bioartificiální kardiovaskulární záplaty a cévní náhrady na bázi porcinního kolagenu zesílené nano/mikrovlákny remodelované pomocí kmenových buněk v bioreaktorech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Advances in Digital Health and Medical Bioengineering, Proceedings of the 11th International Conference on E-Health and Bioengineering, EHB-2023, November 9–10, 2023, Bucharest, Romania – Volume 2: Health Technology Assessment, Biomedical Signal Processing, Medicine and Informatics

ISBN

978-3-031-62519-0

ISSN

1680-0737

e-ISSN

1433-9277

Počet stran výsledku

9

Strana od-do

388-396

Název nakladatele

Springer Nature Switzerland AG

Místo vydání

Basel

Místo konání akce

Bucuresti

Datum konání akce

9. 11. 2023

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

001326809000043