Composite 3D Printed Scaffold with Structured Electrospun Nanofibers Promotes Chondrocyte Adhesion and Infiltration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21720%2F18%3A00317966" target="_blank" >RIV/68407700:21720/18:00317966 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/18:00482687 RIV/46747885:24410/18:00006035 RIV/46747885:24620/18:00006035

Výsledek na webu

<a href="http://dx.doi.org/10.1080/19336918.2017.1385713" target="_blank" >http://dx.doi.org/10.1080/19336918.2017.1385713</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/19336918.2017.1385713" target="_blank" >10.1080/19336918.2017.1385713</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Composite 3D Printed Scaffold with Structured Electrospun Nanofibers Promotes Chondrocyte Adhesion and Infiltration

Popis výsledku v původním jazyce

Additive manufacturing, also called 3D printing, is an effective method for preparing scaffolds with defined structure and porosity. The disadvantage of the technique is the excessive smoothness of the printed fibers, which does not support cell adhesion. In the present study, a 3D printed scaffold was combined with electrospun classic or structured nanofibers to promote cell adhesion. Structured nanofibers were used to improve the infiltration of cells into the scaffold. Electrospun layers were connected to 3D printed fibers by gluing, thus enabling the fabrication of scaffolds with unlimited thickness. The composite 3D printed/nanofibrous scaffolds were seeded with primary chondrocytes and tested in vitro for cell adhesion, proliferation and differentiation. The experiment showed excellent cell infiltration, viability, and good cell proliferation. On the other hand, partial chondrocyte dedifferentiation was shown. Other materials supporting chondrogenic differentiation will be investigated in future studies.

Název v anglickém jazyce

Composite 3D Printed Scaffold with Structured Electrospun Nanofibers Promotes Chondrocyte Adhesion and Infiltration

Popis výsledku anglicky

Additive manufacturing, also called 3D printing, is an effective method for preparing scaffolds with defined structure and porosity. The disadvantage of the technique is the excessive smoothness of the printed fibers, which does not support cell adhesion. In the present study, a 3D printed scaffold was combined with electrospun classic or structured nanofibers to promote cell adhesion. Structured nanofibers were used to improve the infiltration of cells into the scaffold. Electrospun layers were connected to 3D printed fibers by gluing, thus enabling the fabrication of scaffolds with unlimited thickness. The composite 3D printed/nanofibrous scaffolds were seeded with primary chondrocytes and tested in vitro for cell adhesion, proliferation and differentiation. The experiment showed excellent cell infiltration, viability, and good cell proliferation. On the other hand, partial chondrocyte dedifferentiation was shown. Other materials supporting chondrogenic differentiation will be investigated in future studies.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20505 - Composites (including laminates, reinforced plastics, cermets, combined natural and synthetic fibre fabrics; filled composites)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell Adhesion & Migration

ISSN

1933-6918

e-ISSN

1933-6926

Svazek periodika

12

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

271-285

Kód UT WoS článku

000452035500009

EID výsledku v databázi Scopus

2-s2.0-85033731336