Analysis of aflatoxins using miniaturised capillary liquid chromatography system with novel absorbance-fluorescence detector

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70565813%3A_____%2F19%3AN0000041" target="_blank" >RIV/70565813:_____/19:N0000041 - isvavai.cz</a>

Výsledek na webu

<a href="https://repositorium.uni-muenster.de/document/miami/f9538692-6d68-4f89-8785-eb6d0a002c7d/proceedings_bima_2019.pdf" target="_blank" >https://repositorium.uni-muenster.de/document/miami/f9538692-6d68-4f89-8785-eb6d0a002c7d/proceedings_bima_2019.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of aflatoxins using miniaturised capillary liquid chromatography system with novel absorbance-fluorescence detector

Popis výsledku v původním jazyce

Poster at international scientific conference Münster Conference on Biolomecule Analysis, Nov. 6-7, 2019, Germany Aflatoxins are group of fungal pathogens produced by molds of Aspergillus sp. which commonly contaminate cereals, oilseeds, tree nuts, dry fruits and spices. Aflatoxins are toxic substances with immunosuppressive, mutagenic, and carcinogenic effects, and due to their frequent incidence and toxicity they represent health hazard to humans. Several analytical techniques have been used in the past for their detection and quantification in different matrices, including LC/MS, CE/MS as well as HPLC with fluorescence detection [1, 2]. Commercial HPLC/FLD systems are robust but bulky instruments intended for laboratory use. Herein, we introduce miniaturised and portable instrument with low solvent consumption and waste production. The system consists of simplified capillary LC scheme based on the single piston pump and selector valve connected via capillary which serves as a solvent and sample loop [3]. Selector valve allows automated flow path change, such that solvents and sample are sucked by the pump and consequently pumped on capillary column (0.02 × 150 mm packed with Fortis H2O 3m particles). The setup is equipped with the newly developed compact optical detector which is capable of simultaneous multi-wavelength absorbance and fluorescence monitoring. Detector uses light emitting diodes (LEDs, 365 nm, 265 nm) as the light source, lab-made L shaped silica capillary detection cell [4] (50 nL, optical path 1 mm), and CCD spectrometer as the light detector. We applicated this system for analysis of aflatoxins – standard mixture of B1, B2, G1 and G2, as well as for samples obtained from wheat grain inoculated with Aspergillus sp. and Fusarium sp. cultures. Presented results demonstrate the capability of miniaturised capillary liquid chromatograph to perform fast (up to 3.5 min) and repeatable baseline separation of aflatoxins in standard mixture, as well as in complex matrices. The LOD was 0.8 µg/L of G2 and B2, or 6 µg/L of G1 and B1 aflatoxins.

Název v anglickém jazyce

Analysis of aflatoxins using miniaturised capillary liquid chromatography system with novel absorbance-fluorescence detector

Popis výsledku anglicky

Poster at international scientific conference Münster Conference on Biolomecule Analysis, Nov. 6-7, 2019, Germany Aflatoxins are group of fungal pathogens produced by molds of Aspergillus sp. which commonly contaminate cereals, oilseeds, tree nuts, dry fruits and spices. Aflatoxins are toxic substances with immunosuppressive, mutagenic, and carcinogenic effects, and due to their frequent incidence and toxicity they represent health hazard to humans. Several analytical techniques have been used in the past for their detection and quantification in different matrices, including LC/MS, CE/MS as well as HPLC with fluorescence detection [1, 2]. Commercial HPLC/FLD systems are robust but bulky instruments intended for laboratory use. Herein, we introduce miniaturised and portable instrument with low solvent consumption and waste production. The system consists of simplified capillary LC scheme based on the single piston pump and selector valve connected via capillary which serves as a solvent and sample loop [3]. Selector valve allows automated flow path change, such that solvents and sample are sucked by the pump and consequently pumped on capillary column (0.02 × 150 mm packed with Fortis H2O 3m particles). The setup is equipped with the newly developed compact optical detector which is capable of simultaneous multi-wavelength absorbance and fluorescence monitoring. Detector uses light emitting diodes (LEDs, 365 nm, 265 nm) as the light source, lab-made L shaped silica capillary detection cell [4] (50 nL, optical path 1 mm), and CCD spectrometer as the light detector. We applicated this system for analysis of aflatoxins – standard mixture of B1, B2, G1 and G2, as well as for samples obtained from wheat grain inoculated with Aspergillus sp. and Fusarium sp. cultures. Presented results demonstrate the capability of miniaturised capillary liquid chromatograph to perform fast (up to 3.5 min) and repeatable baseline separation of aflatoxins in standard mixture, as well as in complex matrices. The LOD was 0.8 µg/L of G2 and B2, or 6 µg/L of G1 and B1 aflatoxins.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/VI20172020069" target="_blank" >VI20172020069: Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů