Portable liquid chromatograph for on-site analysis of toxins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70565813%3A_____%2F22%3AN0000023" target="_blank" >RIV/70565813:_____/22:N0000023 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.cbrnesymposium.se/program/" target="_blank" >https://www.cbrnesymposium.se/program/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Portable liquid chromatograph for on-site analysis of toxins

Popis výsledku v původním jazyce

Liquid chromatography is widely used technique for non-volatile samples including drugs, environmental pollutants, toxins, chemical warfare agents or their degradation products and metabolites. Current development in miniaturization in last decades has opened new possibilities for construction of portable instruments enabling field analysis at the point of need. Herein, we introduce miniaturised and portable HPLC instrument (miniLC) equipped with a newly developed compact optical detector capable of simultaneous multi-wavelength absorbance and fluorescence monitoring. The system consists of simplified capillary LC scheme based on the single piston pump and selector valve connected via capillary which serves as a solvent and sample loop. Selector valve allows automated flow path change and consequent pumping on capillary column (0.2 × 100 mm packed with Fortis H2O 3mm particles). The detector combines light emitting diodes (LEDs 265 nm and 340 nm, and LEDs 365 nm and 470 nm, respectively) as the light source for absorption measurement and/or fluorescence excitation, respectively, lab-made L shaped silica capillary detection cell (50 nL, optical path 1 mm) [1], and CCD spectrometer as the light detector. We applied this system for analysis of toxins (aflatoxins and microcystins) and selected CWA surrogates. Presented results demonstrate the capability of miniaturised capillary liquid chromatograph to perform fast (up to 3.5 min) and repeatable separation of aflatoxins in standard mixture and/or in complex matrices, as well as fast separations of other toxic agents. The research follows with application of the portable miniLC for other hazardous agents of concern.

Název v anglickém jazyce

Portable liquid chromatograph for on-site analysis of toxins

Popis výsledku anglicky

Liquid chromatography is widely used technique for non-volatile samples including drugs, environmental pollutants, toxins, chemical warfare agents or their degradation products and metabolites. Current development in miniaturization in last decades has opened new possibilities for construction of portable instruments enabling field analysis at the point of need. Herein, we introduce miniaturised and portable HPLC instrument (miniLC) equipped with a newly developed compact optical detector capable of simultaneous multi-wavelength absorbance and fluorescence monitoring. The system consists of simplified capillary LC scheme based on the single piston pump and selector valve connected via capillary which serves as a solvent and sample loop. Selector valve allows automated flow path change and consequent pumping on capillary column (0.2 × 100 mm packed with Fortis H2O 3mm particles). The detector combines light emitting diodes (LEDs 265 nm and 340 nm, and LEDs 365 nm and 470 nm, respectively) as the light source for absorption measurement and/or fluorescence excitation, respectively, lab-made L shaped silica capillary detection cell (50 nL, optical path 1 mm) [1], and CCD spectrometer as the light detector. We applied this system for analysis of toxins (aflatoxins and microcystins) and selected CWA surrogates. Presented results demonstrate the capability of miniaturised capillary liquid chromatograph to perform fast (up to 3.5 min) and repeatable separation of aflatoxins in standard mixture and/or in complex matrices, as well as fast separations of other toxic agents. The research follows with application of the portable miniLC for other hazardous agents of concern.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/VI04000062" target="_blank" >VI04000062: Miniaturizace kapalinového chromatografu – rozšíření aplikačního spektra pro detekci a stanovení nebezpečných látek v podmínkách mobilní laboratoře</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů