Characterization of Escherichia Coli Strains Isolated from Food

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of Escherichia Coli Strains Isolated from Food

Popis výsledku v původním jazyce

The objective of this study was to determine current state of antibiotic resistance and the presence of virulence factors among a collection of 69 Escherichia coli strains isolated from retail meat in the Czech Republic. The incidence of antibiotic resistance, presence of 14 virulence factors and production of 23 colicins and 5 microcins were tested after classification into phylogenetic groups. The antimicrobial resistance of E. coli strains was detected by using disk diffusion test. The multiplex PCRmethod was used for determination of phylogenetic groups and single PCR method for bacteriocinotyping of producer strains and presence of virulence factors. In this study, almost 48 % of all E. coli strains were resistant to at east one of 8 tested antibiotics and 6% strains had a multidrug resistance. The higher rate of antibiotic resistance was to ampicillin and amoxicillin / clavulanic acid. More than half strains belonged to A phylogroup. The other strains were lassified into B2 and

Název v anglickém jazyce

Characterization of Escherichia Coli Strains Isolated from Food

Popis výsledku anglicky

The objective of this study was to determine current state of antibiotic resistance and the presence of virulence factors among a collection of 69 Escherichia coli strains isolated from retail meat in the Czech Republic. The incidence of antibiotic resistance, presence of 14 virulence factors and production of 23 colicins and 5 microcins were tested after classification into phylogenetic groups. The antimicrobial resistance of E. coli strains was detected by using disk diffusion test. The multiplex PCRmethod was used for determination of phylogenetic groups and single PCR method for bacteriocinotyping of producer strains and presence of virulence factors. In this study, almost 48 % of all E. coli strains were resistant to at east one of 8 tested antibiotics and 6% strains had a multidrug resistance. The higher rate of antibiotic resistance was to ampicillin and amoxicillin / clavulanic acid. More than half strains belonged to A phylogroup. The other strains were lassified into B2 and

Druh

D - Stať ve sborníku

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

The 4th International Scientific Conference Applied Natural Sciences 2013 APV TRNAVA 2013

ISBN

978-80-8105-502-7

ISSN

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e-ISSN

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Počet stran výsledku

8

Strana od-do

195-202

Název nakladatele

Univerzita sv. Cyrila a Metoda v Trnave

Místo vydání

Trnava

Místo konání akce

Nový Smokovec

Datum konání akce

2. 10. 2013

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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