Escherichia coli isolated from food as a risk factor to human health

Kód výsledku v IS VaVaI

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RIV/70883521:28610/19:63523307

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Escherichia coli isolated from food as a risk factor to human health

Popis výsledku v původním jazyce

The Escherichia coli is considered heterogeneous species classified into four main phylogenetic groups A, B1, B2, and D which differ in genome sizes, virulence and antibiotic resistance profiles. To compete with other bacteria,many E. coli strains synthesize antimicrobial agents (colicins, microcins). The ecological role of bacteriocinogeny remains unclear. Whereas commensal strains help maintaining the balance in gastrointestinal tract, pathogenic strains can cause a broad range of human diseases including food poisoning. Objectives: The aim of this study was to investigate the potential threat of Escherichia coli strains isolated from various food to human health. Methods: E. coli strains originating from food (chicken, pork, and game meat, cheese, vegetables, cakes) were phenotypically examined for bacteriocin production and antibiotic resistance (EUCAST), followed by PCR detection of individual bacteriocin, antibiotic resistance, and virulence factor genes. In addition, whole genome sequencing (WGS) was also performed. Results: Altogether, 160 E. coli strains were examined in this study. The most common resistances observed were against aminopenicillins; resistance to fluoroquinolones and aminoglycosides was also prevalent. Among isolated strains, 37% of bacteriocinogeny incidence (colicins: E7, E1, B, M, Ia; microcins: mV, mC7, mB17) was detected. ETEC and EIEC virulence factors were found in game meat and vegetables strains, on the other hand, APEC, UPEC and ExPEC virulence factors were proved in chicken meat and cheese strains. WGS generally confirmed the results detected by PCR. Thus, food can be a source of alimentary infection (game meat, vegetables) and also can be a vehicle of antibiotic resistance spreading.

Název v anglickém jazyce

Escherichia coli isolated from food as a risk factor to human health

Popis výsledku anglicky

The Escherichia coli is considered heterogeneous species classified into four main phylogenetic groups A, B1, B2, and D which differ in genome sizes, virulence and antibiotic resistance profiles. To compete with other bacteria,many E. coli strains synthesize antimicrobial agents (colicins, microcins). The ecological role of bacteriocinogeny remains unclear. Whereas commensal strains help maintaining the balance in gastrointestinal tract, pathogenic strains can cause a broad range of human diseases including food poisoning. Objectives: The aim of this study was to investigate the potential threat of Escherichia coli strains isolated from various food to human health. Methods: E. coli strains originating from food (chicken, pork, and game meat, cheese, vegetables, cakes) were phenotypically examined for bacteriocin production and antibiotic resistance (EUCAST), followed by PCR detection of individual bacteriocin, antibiotic resistance, and virulence factor genes. In addition, whole genome sequencing (WGS) was also performed. Results: Altogether, 160 E. coli strains were examined in this study. The most common resistances observed were against aminopenicillins; resistance to fluoroquinolones and aminoglycosides was also prevalent. Among isolated strains, 37% of bacteriocinogeny incidence (colicins: E7, E1, B, M, Ia; microcins: mV, mC7, mB17) was detected. ETEC and EIEC virulence factors were found in game meat and vegetables strains, on the other hand, APEC, UPEC and ExPEC virulence factors were proved in chicken meat and cheese strains. WGS generally confirmed the results detected by PCR. Thus, food can be a source of alimentary infection (game meat, vegetables) and also can be a vehicle of antibiotic resistance spreading.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

21101 - Food and beverages

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů