Characterization of Escherichia coli strains isolated from raw vegetables
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F18%3A63520418" target="_blank" >RIV/70883521:28110/18:63520418 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.5219/897" target="_blank" >http://dx.doi.org/10.5219/897</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5219/897" target="_blank" >10.5219/897</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Characterization of Escherichia coli strains isolated from raw vegetables
Popis výsledku v původním jazyce
Vegetables are an important part of the human diet. Sometimes, contamination by pathogenic Escherichia coli can be underestimated; moreover there is a risk of antibiotic resistance spreading via the food chain. The purpose of this study was to examine the prevalence of Escherichia coli in fresh vegetables sold in retail market in the Czech Republic and to evaluate the risk to human health. Antibiotic resistance against 12 antibiotics, the presence of 12 virulence and 15 resistance genes were determined among 15 isolated strains. Most of tested strains belonged to B1 phylogenetic group, less frequently represented was B2 and D phylogroup. These results indicate that most strains are probably of human origin. All E. coli strains were resistant to at least one of twelve tested antibiotics. A multidrug resistance was observed in four strains. In this study, the presence of virulence factors Einv and papC and also genes encoding toxins (CNF1, CNF2) was detected. Nevertheless, none strain can be considered as STEC or EHEC. The widespread appearance of a growing trend associated with the prevalence of antibiotic resistance among enterobacterial isolates is undeniable and the possibility of transfer to humans cannot be ignored. Nevertheless, these results indicate that raw vegetables sold in the retail market can constitute a potential health risk for consumers.
Název v anglickém jazyce
Characterization of Escherichia coli strains isolated from raw vegetables
Popis výsledku anglicky
Vegetables are an important part of the human diet. Sometimes, contamination by pathogenic Escherichia coli can be underestimated; moreover there is a risk of antibiotic resistance spreading via the food chain. The purpose of this study was to examine the prevalence of Escherichia coli in fresh vegetables sold in retail market in the Czech Republic and to evaluate the risk to human health. Antibiotic resistance against 12 antibiotics, the presence of 12 virulence and 15 resistance genes were determined among 15 isolated strains. Most of tested strains belonged to B1 phylogenetic group, less frequently represented was B2 and D phylogroup. These results indicate that most strains are probably of human origin. All E. coli strains were resistant to at least one of twelve tested antibiotics. A multidrug resistance was observed in four strains. In this study, the presence of virulence factors Einv and papC and also genes encoding toxins (CNF1, CNF2) was detected. Nevertheless, none strain can be considered as STEC or EHEC. The widespread appearance of a growing trend associated with the prevalence of antibiotic resistance among enterobacterial isolates is undeniable and the possibility of transfer to humans cannot be ignored. Nevertheless, these results indicate that raw vegetables sold in the retail market can constitute a potential health risk for consumers.
Klasifikace
Druh
J<sub>SC</sub> - Článek v periodiku v databázi SCOPUS
CEP obor
—
OECD FORD obor
40500 - Other agricultural sciences
Návaznosti výsledku
Projekt
—
Návaznosti
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Potravinárstvo
ISSN
1338-0230
e-ISSN
—
Svazek periodika
12
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
SK - Slovenská republika
Počet stran výsledku
9
Strana od-do
304-312
Kód UT WoS článku
—
EID výsledku v databázi Scopus
2-s2.0-85044616745