Microflora of farm and hunted pheasants in relation to biogenic amines production

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F16%3A43874549" target="_blank" >RIV/70883521:28110/16:43874549 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007/s10344-016-1008-y" target="_blank" >http://link.springer.com/article/10.1007/s10344-016-1008-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10344-016-1008-y" target="_blank" >10.1007/s10344-016-1008-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Microflora of farm and hunted pheasants in relation to biogenic amines production

Popis výsledku v původním jazyce

The aim of the study was to compare, within 3-week cold storage, the content of eight biogenic amines (BA) and the development of microflora in different parts of bodies (breast and thigh muscles and liver) of farm pheasants and pheasants hunted in the wild. Killed and untreated bodies of pheasants were stored hanging in an air-conditioned chamber at a cold temperature (5 +- 1 oC). The pheasant bodies were subjected to microbiological analysis and identification of isolated microorganisms whose decarboxylase activity was detected. The production of biogenic amines and polyamines was determined by means of HPLC after pre-column derivatisation with dansylchloride. The total number of microorganisms in all parts of the pheasant bodies as well as the number of psychrotrophic bacteria, enterobacteria and lactic acid bacteria was increasing during storage (P < 0.05). A total of 127 strains of bacteria from the pheasant bodies were isolated and identified. Production of at least one biogenic amine was found in vitro in 107 isolates. Three strains were found to produce four biogenic amines-histamine, tyramine, putrescine and cadaverine. Production of phenylethylamine, tryptamine, spermidine and spermine in the isolated bacteria was not detected. Within all the biogenic amines detected in the analysed samples of pheasant bodies, the lowest concentration was found in histamine and the highest one in tyramine and cadaverine. The amount of BA detected in the individual parts of pheasant bodies was increasing during the storage period. The total amounts of seven biogenic amines (histamine, tyramine, putrescine, cadaverine, phenylethylamine, spermidine and spermine) in the individual parts of pheasant bodies were ranging between 9 and 708 mg/kg.

Název v anglickém jazyce

Microflora of farm and hunted pheasants in relation to biogenic amines production

Popis výsledku anglicky

The aim of the study was to compare, within 3-week cold storage, the content of eight biogenic amines (BA) and the development of microflora in different parts of bodies (breast and thigh muscles and liver) of farm pheasants and pheasants hunted in the wild. Killed and untreated bodies of pheasants were stored hanging in an air-conditioned chamber at a cold temperature (5 +- 1 oC). The pheasant bodies were subjected to microbiological analysis and identification of isolated microorganisms whose decarboxylase activity was detected. The production of biogenic amines and polyamines was determined by means of HPLC after pre-column derivatisation with dansylchloride. The total number of microorganisms in all parts of the pheasant bodies as well as the number of psychrotrophic bacteria, enterobacteria and lactic acid bacteria was increasing during storage (P < 0.05). A total of 127 strains of bacteria from the pheasant bodies were isolated and identified. Production of at least one biogenic amine was found in vitro in 107 isolates. Three strains were found to produce four biogenic amines-histamine, tyramine, putrescine and cadaverine. Production of phenylethylamine, tryptamine, spermidine and spermine in the isolated bacteria was not detected. Within all the biogenic amines detected in the analysed samples of pheasant bodies, the lowest concentration was found in histamine and the highest one in tyramine and cadaverine. The amount of BA detected in the individual parts of pheasant bodies was increasing during the storage period. The total amounts of seven biogenic amines (histamine, tyramine, putrescine, cadaverine, phenylethylamine, spermidine and spermine) in the individual parts of pheasant bodies were ranging between 9 and 708 mg/kg.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP503%2F11%2F1417" target="_blank" >GAP503/11/1417: Produkce biogenních aminů u vybraných kmenů bakterií mléčného kvašení</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Wildlife Research

ISSN

1612-4642

e-ISSN

—

Svazek periodika

62

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

12

Strana od-do

341-352

Kód UT WoS článku

000376592300010

EID výsledku v databázi Scopus

2-s2.0-84962191119