3D printing of silk/polycaprolactone composite scaffolds

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F22%3A63557719" target="_blank" >RIV/70883521:28110/22:63557719 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28610/22:63557719

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

3D printing of silk/polycaprolactone composite scaffolds

Popis výsledku v původním jazyce

Silk protein is a biocompatible material with good mechanical properties, suitable for the development of 3D scaffolds and tissue engineering applications. The conformational transition of the secondary structure of silk fibroin due to shear stress complicates its processability using 3D printing techniques. Therefore, the solution of fibroin with polycaprolactone (PCL) in hexafluoroisopropanol as a good solvent and dimethylsulfoxid as a poor solvent were prepared. From these mixtures, 3D objects were prepared by extrusion printing and the arrangements of macromolecules in the printouts were analyzed. Using atomic force microscopy (AFM), we observed formation of polycaprolactone spherulites which were disrupted by the incorporated fibroin in the form of nano- to micro-particulate structures. The organization of the lamellae of the PCL spherulites and topography of the printed structures was rearranged by a phase separation process caused by the poor solvent in the printing dope. Resulting PCL/Fibroin scaffolds revealed good biocompatibility and mechanical stability.

Název v anglickém jazyce

3D printing of silk/polycaprolactone composite scaffolds

Popis výsledku anglicky

Silk protein is a biocompatible material with good mechanical properties, suitable for the development of 3D scaffolds and tissue engineering applications. The conformational transition of the secondary structure of silk fibroin due to shear stress complicates its processability using 3D printing techniques. Therefore, the solution of fibroin with polycaprolactone (PCL) in hexafluoroisopropanol as a good solvent and dimethylsulfoxid as a poor solvent were prepared. From these mixtures, 3D objects were prepared by extrusion printing and the arrangements of macromolecules in the printouts were analyzed. Using atomic force microscopy (AFM), we observed formation of polycaprolactone spherulites which were disrupted by the incorporated fibroin in the form of nano- to micro-particulate structures. The organization of the lamellae of the PCL spherulites and topography of the printed structures was rearranged by a phase separation process caused by the poor solvent in the printing dope. Resulting PCL/Fibroin scaffolds revealed good biocompatibility and mechanical stability.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

<a href="/cs/project/GA22-33307S" target="_blank" >GA22-33307S: Vývoj nových 3D hierarchicky strukturovaných polysacharidových a proteinových porézních systémů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů