Whey protein isolate-chitosan PolyElectrolyte Nanoparticles as a drug delivery system

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F23%3A63566818" target="_blank" >RIV/70883521:28110/23:63566818 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28610/23:63566818

Výsledek na webu

<a href="https://www.mdpi.com/1420-3049/28/4/1724" target="_blank" >https://www.mdpi.com/1420-3049/28/4/1724</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/molecules28041724" target="_blank" >10.3390/molecules28041724</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Whey protein isolate-chitosan PolyElectrolyte Nanoparticles as a drug delivery system

Popis výsledku v původním jazyce

Whey protein isolate (WPI), employed as a carrier for a wide range of bioactive substances, suffers from a lack of colloidal stability in physiological conditions. Herein, we developed innovative stabilized PolyElectrolyte Nanoparticles (PENs) obtained by two techniques: polyelectrolyte complexation of negatively charged WPI and positively charged chitosan (CS), and ionic gelation in the presence of polyanion tripolyphosphate (TPP). Therefore, the WPI-based core was coated with a CS-based shell and then stabilized by TPP at pH 8. The nanostructures were characterized by physiochemical methods, and their encapsulation efficiency and in vitro release were evaluated. The spherical NPs with an average size of 248.57 +/- 5.00 nm and surface charge of +10.80 +/- 0.43 mV demonstrated high encapsulation efficiency (92.79 +/- 0.69) and sustained release of a positively charged chemotherapeutic agent such as doxorubicin (DOX). Z-average size and size distribution also presented negligible increases in size and aggregates during the three weeks. The results obtained confirm the effectiveness of the simultaneous application of these methods to improve the colloidal stability of PEN.

Název v anglickém jazyce

Whey protein isolate-chitosan PolyElectrolyte Nanoparticles as a drug delivery system

Popis výsledku anglicky

Whey protein isolate (WPI), employed as a carrier for a wide range of bioactive substances, suffers from a lack of colloidal stability in physiological conditions. Herein, we developed innovative stabilized PolyElectrolyte Nanoparticles (PENs) obtained by two techniques: polyelectrolyte complexation of negatively charged WPI and positively charged chitosan (CS), and ionic gelation in the presence of polyanion tripolyphosphate (TPP). Therefore, the WPI-based core was coated with a CS-based shell and then stabilized by TPP at pH 8. The nanostructures were characterized by physiochemical methods, and their encapsulation efficiency and in vitro release were evaluated. The spherical NPs with an average size of 248.57 +/- 5.00 nm and surface charge of +10.80 +/- 0.43 mV demonstrated high encapsulation efficiency (92.79 +/- 0.69) and sustained release of a positively charged chemotherapeutic agent such as doxorubicin (DOX). Z-average size and size distribution also presented negligible increases in size and aggregates during the three weeks. The results obtained confirm the effectiveness of the simultaneous application of these methods to improve the colloidal stability of PEN.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecules

ISSN

1420-3049

e-ISSN

—

Svazek periodika

28

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

—

Kód UT WoS článku

000941722100001

EID výsledku v databázi Scopus

2-s2.0-85149053047