Preparation and characterization of gelatin-based porous systems using X-ray tomography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F23%3A63571141" target="_blank" >RIV/70883521:28110/23:63571141 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28610/23:63571141

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Preparation and characterization of gelatin-based porous systems using X-ray tomography

Popis výsledku v původním jazyce

3D printing of biocompatible materials based on gelatin is one of the possible ways to successfully create structures capable of supporting cells and ensuring their proliferation. The mechanical properties of pure gelatin are not suitable for additive manufacturing and it is necessary to improve them, for example, by cross-linking, where the Schiff base method was chosen as the most suitable. In the experiments, gelatin (rabbit and bovine) was mixed with oxidized dextran in different ratios (1 to 1, 1 to 2, 1 to 3) with the addition of activating agents (NH3 and TEA). The resulting material was then printed on a 3D bioprinter BIO X (Cellink) in layers (1, 3, 5) with a predefined pattern. These scaffolds were subsequently subjected to porosity analysis in both the native and lyophilized states using scanning electron microscopy and computed tomography. The results confirm the theory that the prepared material could serve as a support material for printing cells in tissue engineering in the future.

Název v anglickém jazyce

Preparation and characterization of gelatin-based porous systems using X-ray tomography

Popis výsledku anglicky

3D printing of biocompatible materials based on gelatin is one of the possible ways to successfully create structures capable of supporting cells and ensuring their proliferation. The mechanical properties of pure gelatin are not suitable for additive manufacturing and it is necessary to improve them, for example, by cross-linking, where the Schiff base method was chosen as the most suitable. In the experiments, gelatin (rabbit and bovine) was mixed with oxidized dextran in different ratios (1 to 1, 1 to 2, 1 to 3) with the addition of activating agents (NH3 and TEA). The resulting material was then printed on a 3D bioprinter BIO X (Cellink) in layers (1, 3, 5) with a predefined pattern. These scaffolds were subsequently subjected to porosity analysis in both the native and lyophilized states using scanning electron microscopy and computed tomography. The results confirm the theory that the prepared material could serve as a support material for printing cells in tissue engineering in the future.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10404 - Polymer science

Projekt

<a href="/cs/project/GA22-33307S" target="_blank" >GA22-33307S: Vývoj nových 3D hierarchicky strukturovaných polysacharidových a proteinových porézních systémů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů