Fabrication of textured silk films via phase-separation approach for applica-tions in regenerative medicine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F23%3A63571159" target="_blank" >RIV/70883521:28110/23:63571159 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28610/23:63571159

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fabrication of textured silk films via phase-separation approach for applica-tions in regenerative medicine

Popis výsledku v původním jazyce

The poor cell adhesion properties of silk protein (Bombyx mori) surfaces require additional surface treatments to increase the adhesive capabilities of selected cell lines. In this research, coatings made of the silk protein were structured using a dosed addition of solvent/non-solvent mixtures. To control over the surface modification process, we developed a specialized device that allows precise setting of process parameters, such as time delays or dosing volumes of droplets. By optimizing the process parameters for surface modification, we successfully controlled the formation of nano- to microporous structures on the silk surfaces. The surface modification also significantly depended on the initial secondary conformations of the protein i.e. content of beta-sheet structures. The textured surfaces exhibited improved properties for adhesion and proliferation of diverse cell lines, thus greatly expanding the potential applications of silk-based materials in the field of regenerative medicine.

Název v anglickém jazyce

Fabrication of textured silk films via phase-separation approach for applica-tions in regenerative medicine

Popis výsledku anglicky

The poor cell adhesion properties of silk protein (Bombyx mori) surfaces require additional surface treatments to increase the adhesive capabilities of selected cell lines. In this research, coatings made of the silk protein were structured using a dosed addition of solvent/non-solvent mixtures. To control over the surface modification process, we developed a specialized device that allows precise setting of process parameters, such as time delays or dosing volumes of droplets. By optimizing the process parameters for surface modification, we successfully controlled the formation of nano- to microporous structures on the silk surfaces. The surface modification also significantly depended on the initial secondary conformations of the protein i.e. content of beta-sheet structures. The textured surfaces exhibited improved properties for adhesion and proliferation of diverse cell lines, thus greatly expanding the potential applications of silk-based materials in the field of regenerative medicine.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20501 - Materials engineering

Projekt

<a href="/cs/project/GA22-33307S" target="_blank" >GA22-33307S: Vývoj nových 3D hierarchicky strukturovaných polysacharidových a proteinových porézních systémů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů