Hierarchical nano- and microstructuring of biopolymer surfaces

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F24%3A63583858" target="_blank" >RIV/70883521:28110/24:63583858 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Hierarchical nano- and microstructuring of biopolymer surfaces

Popis výsledku v původním jazyce

We present a novel approach to modifications of Silk protein (Bombyx mori) and hyaluronan surfaces by a solvent induced phase separation. In this research, coatings made of the biopolymers were structured using a dosed addition of solvent/non-solvent mixtures. To control the surface modification process, we developed a specialized device that allows precise setting of process parameters, such as time delays or dosing volumes of droplets. By optimizing the process parameters as well as the composition of solvent/non-solvent mixtures the formation of various nano- to microporous structures on the silk or hyaluronan surfaces have been achieved. The textures of the biomaterials coatings were significantly determined by the initial secondary conformations of the fibroin, i.e., content of beta-sheet structures or hydration of the hyaluronan surface layer. The textured silk surfaces exhibited improved properties for adhesion and proliferation of diverse cell lines, thus greatly expanding the potential applications of silk-based materials in the field of regenerative medicine. The textured hyaluronan film could be employed for the preparation of drug carriers with improved mechanical properties and accelerated solubility.

Název v anglickém jazyce

Hierarchical nano- and microstructuring of biopolymer surfaces

Popis výsledku anglicky

We present a novel approach to modifications of Silk protein (Bombyx mori) and hyaluronan surfaces by a solvent induced phase separation. In this research, coatings made of the biopolymers were structured using a dosed addition of solvent/non-solvent mixtures. To control the surface modification process, we developed a specialized device that allows precise setting of process parameters, such as time delays or dosing volumes of droplets. By optimizing the process parameters as well as the composition of solvent/non-solvent mixtures the formation of various nano- to microporous structures on the silk or hyaluronan surfaces have been achieved. The textures of the biomaterials coatings were significantly determined by the initial secondary conformations of the fibroin, i.e., content of beta-sheet structures or hydration of the hyaluronan surface layer. The textured silk surfaces exhibited improved properties for adhesion and proliferation of diverse cell lines, thus greatly expanding the potential applications of silk-based materials in the field of regenerative medicine. The textured hyaluronan film could be employed for the preparation of drug carriers with improved mechanical properties and accelerated solubility.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20501 - Materials engineering

Projekt

<a href="/cs/project/GA22-33307S" target="_blank" >GA22-33307S: Vývoj nových 3D hierarchicky strukturovaných polysacharidových a proteinových porézních systémů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů