Raman spectroscopy as a modern tool for lactose determination

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28140%2F16%3A43874830" target="_blank" >RIV/70883521:28140/16:43874830 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.wseas.org/multimedia/journals/biology/2016/a265808-081.pdf" target="_blank" >http://www.wseas.org/multimedia/journals/biology/2016/a265808-081.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Raman spectroscopy as a modern tool for lactose determination

Popis výsledku v původním jazyce

Digestion of lactose, the milk disaccharide, makes problem to a large number of the population. Therefore, the lactose intolerant individuals are limited in the intake of milk and dairy products. The lactose -free products offers a solution for the diet. Raman spectroscopic analyses were performed for a purpose of rapid assessment of lactose content in bovine milk, lactose-free milk, mixtures of bovine milk and lactose-free milk, and bovine milk with additions of lactose. Based on characteristic vibrations, Raman spectra of lactose, glucose and galactose exhibit enough differences to distinguish the content of lactose in milk. C-O-H bending mode at 1087 cm-1 is used for lactose quantification. Two different substances - phenylalanine contained generally in the milk and crystal violet used as an internal standard, were studied for the evaluation of the spectroscopic data. The content of phenylalanine in samples was verified by IEC. Better accuracy exhibits the phenylalanine normalization. The contents of lactose and more easily digestible monosaccharides - glucose and galactose from the mixtures of milk and lactose-free milk were analysed by HPLC, Raman spectroscopy provided corresponding results. The study shows that Raman spectroscopy is an effective method for the feedback control of lactose.

Název v anglickém jazyce

Raman spectroscopy as a modern tool for lactose determination

Popis výsledku anglicky

Digestion of lactose, the milk disaccharide, makes problem to a large number of the population. Therefore, the lactose intolerant individuals are limited in the intake of milk and dairy products. The lactose -free products offers a solution for the diet. Raman spectroscopic analyses were performed for a purpose of rapid assessment of lactose content in bovine milk, lactose-free milk, mixtures of bovine milk and lactose-free milk, and bovine milk with additions of lactose. Based on characteristic vibrations, Raman spectra of lactose, glucose and galactose exhibit enough differences to distinguish the content of lactose in milk. C-O-H bending mode at 1087 cm-1 is used for lactose quantification. Two different substances - phenylalanine contained generally in the milk and crystal violet used as an internal standard, were studied for the evaluation of the spectroscopic data. The content of phenylalanine in samples was verified by IEC. Better accuracy exhibits the phenylalanine normalization. The contents of lactose and more easily digestible monosaccharides - glucose and galactose from the mixtures of milk and lactose-free milk were analysed by HPLC, Raman spectroscopy provided corresponding results. The study shows that Raman spectroscopy is an effective method for the feedback control of lactose.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

WSEAS Transactions on Biology and Biomedicine

ISSN

1109-9518

e-ISSN

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Svazek periodika

13

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

7

Strana od-do

108-114

Kód UT WoS článku

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EID výsledku v databázi Scopus

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