Self-crosslinked semi-interpenetrated polysaccharide-based gels for the controlled delivery of antibiotic drugs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F22%3A63560558" target="_blank" >RIV/70883521:28610/22:63560558 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Self-crosslinked semi-interpenetrated polysaccharide-based gels for the controlled delivery of antibiotic drugs

Popis výsledku v původním jazyce

Antibiotics are well known to be use for systemic therapy via parenteral as well as topical administration for bacterial infections. However, owing to their short half-life, most antibiotics and the conventional drug delivery systems, limits the pharmacokinetic profile of the drugs. This may display toxicity and narrow therapeutic effect to the targeted areas. This study reports the preparation and physicochemical characterization of chitosan crosslinked dialdehyde xanthan gum gels semi-interpenetrated with hydroxypropyl methylcellulose for the controlled delivery of minocycline and rifampicin antibiotic drugs. The prepared gels were characterized by FTIR, SEM, TGA, mechanical and rheology methods. In addition, gelation kinetics, swelling, degradation and drug release were studied under simulated stomach and plasma physiological conditions of pH 2.0 and 7.4, respectively, at 37 °C. The results showed more than 50% cumulative release within 24 h for all investigated antibiotic drugs. Cytocompatibility by MIT assay using mouse embryonic fibroblast cell lines were tested and cell viability was determined to be ????80%, confirrning the gels are non-toxic. Finally, the antibacterial effect of loaded gels was evaluated against Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia). The observed antibacterial effect correlated well with swelling and drug release results. Overall, our results demonstrated the produced gels as promising materials for controlled delivery drug molecules for biomedical applications.

Název v anglickém jazyce

Self-crosslinked semi-interpenetrated polysaccharide-based gels for the controlled delivery of antibiotic drugs

Popis výsledku anglicky

Antibiotics are well known to be use for systemic therapy via parenteral as well as topical administration for bacterial infections. However, owing to their short half-life, most antibiotics and the conventional drug delivery systems, limits the pharmacokinetic profile of the drugs. This may display toxicity and narrow therapeutic effect to the targeted areas. This study reports the preparation and physicochemical characterization of chitosan crosslinked dialdehyde xanthan gum gels semi-interpenetrated with hydroxypropyl methylcellulose for the controlled delivery of minocycline and rifampicin antibiotic drugs. The prepared gels were characterized by FTIR, SEM, TGA, mechanical and rheology methods. In addition, gelation kinetics, swelling, degradation and drug release were studied under simulated stomach and plasma physiological conditions of pH 2.0 and 7.4, respectively, at 37 °C. The results showed more than 50% cumulative release within 24 h for all investigated antibiotic drugs. Cytocompatibility by MIT assay using mouse embryonic fibroblast cell lines were tested and cell viability was determined to be ????80%, confirrning the gels are non-toxic. Finally, the antibacterial effect of loaded gels was evaluated against Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia). The observed antibacterial effect correlated well with swelling and drug release results. Overall, our results demonstrated the produced gels as promising materials for controlled delivery drug molecules for biomedical applications.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Projekt

<a href="/cs/project/EF16_028%2F0006243" target="_blank" >EF16_028/0006243: Rozvoj kapacit pro výzkum a vývoj UTB ve Zlíně</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů