SPIDIA-DNA: An External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F13%3A00398939" target="_blank" >RIV/86652036:_____/13:00398939 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/86652036:_____/13:00420963
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.cca.2013.05.012" target="_blank" >http://dx.doi.org/10.1016/j.cca.2013.05.012</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cca.2013.05.012" target="_blank" >10.1016/j.cca.2013.05.012</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
SPIDIA-DNA: An External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses
Popis výsledku v původním jazyce
Background: The EC-funded project SPIDIA is aimed to develop evidence-based quality guidelines for the pre-analytical phase of blood samples used for DNA molecular testing. To this purpose, a survey and a pan-European External Quality Assessment (EQA) were implemented. Methods: SPIDIA facility sent to all the participants the same blood sample to be processed without time or temperature limitation. DNA quality parameters performed at SPIDIA facility included: UV spectrophotometric analysis of DNA purityand yield, PCR interferences study by Kineret software and DNA integrity analysis by pulsed field gel electrophoresis. Results: 197 applications have been collected from 30 European countries. A high variability of DNA fragmentation was observed whereaspurity, yield and PCR interferences had a narrow distribution within laboratories. A significant difference between the RNase P single copy gene quantity obtained in the DNA samples extracted with the precipitation-based method respect t
Název v anglickém jazyce
SPIDIA-DNA: An External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses
Popis výsledku anglicky
Background: The EC-funded project SPIDIA is aimed to develop evidence-based quality guidelines for the pre-analytical phase of blood samples used for DNA molecular testing. To this purpose, a survey and a pan-European External Quality Assessment (EQA) were implemented. Methods: SPIDIA facility sent to all the participants the same blood sample to be processed without time or temperature limitation. DNA quality parameters performed at SPIDIA facility included: UV spectrophotometric analysis of DNA purityand yield, PCR interferences study by Kineret software and DNA integrity analysis by pulsed field gel electrophoresis. Results: 197 applications have been collected from 30 European countries. A high variability of DNA fragmentation was observed whereaspurity, yield and PCR interferences had a narrow distribution within laboratories. A significant difference between the RNase P single copy gene quantity obtained in the DNA samples extracted with the precipitation-based method respect t
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Clinica Chimica Acta
ISSN
0009-8981
e-ISSN
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Svazek periodika
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Číslo periodika v rámci svazku
424
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
13
Strana od-do
274-286
Kód UT WoS článku
000325042600050
EID výsledku v databázi Scopus
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