Semi-empirical description of a retention of small organic probes on AtlantisTM Premier BEH C18 AX mixed-mode column under varying pH and organic modifier conditions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F23%3A00574538" target="_blank" >RIV/86652036:_____/23:00574538 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/23:10470979

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0026265X23005283?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0026265X23005283?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2023.108910" target="_blank" >10.1016/j.microc.2023.108910</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Semi-empirical description of a retention of small organic probes on AtlantisTM Premier BEH C18 AX mixed-mode column under varying pH and organic modifier conditions

Popis výsledku v původním jazyce

Mixed-mode chromatography is a widely used analytical method that utilizes multiple separation mechanisms in one chromatographic column, frequently a reversed-phase and ion-exchange interactions. Adequate understanding of how the individual interactions influence the overall retention of the analyte is important for fast and effective design of mixed-mode-based methods. Previous attempts to derive semi-empirical mathematical models of mixed-mode chromatography were limited to a narrow range of analytes and did not consider the effect of organic modifier in the mobile phase. In this paper, we attempt to improve upon the previous models by extending the range of classes of analytes to weak acids and bases and including the effect of organic modifier. An improved semi-empirical model was derived that captures all important retention trends of the combined effect of reversed-phase and ion-exchange retention mechanisms for all tested classes of analytes and up to 30% (v/v) organic modifier content.

Název v anglickém jazyce

Semi-empirical description of a retention of small organic probes on AtlantisTM Premier BEH C18 AX mixed-mode column under varying pH and organic modifier conditions

Popis výsledku anglicky

Mixed-mode chromatography is a widely used analytical method that utilizes multiple separation mechanisms in one chromatographic column, frequently a reversed-phase and ion-exchange interactions. Adequate understanding of how the individual interactions influence the overall retention of the analyte is important for fast and effective design of mixed-mode-based methods. Previous attempts to derive semi-empirical mathematical models of mixed-mode chromatography were limited to a narrow range of analytes and did not consider the effect of organic modifier in the mobile phase. In this paper, we attempt to improve upon the previous models by extending the range of classes of analytes to weak acids and bases and including the effect of organic modifier. An improved semi-empirical model was derived that captures all important retention trends of the combined effect of reversed-phase and ion-exchange retention mechanisms for all tested classes of analytes and up to 30% (v/v) organic modifier content.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA20-19655S" target="_blank" >GA20-19655S: Strategie pro on-line štěpení proteinů s následnou separací v mix-mode chromatografii</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchemical Journal

ISSN

0026-265X

e-ISSN

1095-9149

Svazek periodika

191

Číslo periodika v rámci svazku

AUG 2023

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

108910

Kód UT WoS článku

001012911400001

EID výsledku v databázi Scopus

2-s2.0-85160699961