Utilization of SSR markers for identification of gene resources of small-leaved linden reproductive material

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00020702%3A_____%2F20%3AN0000140" target="_blank" >RIV/00020702:_____/20:N0000140 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.vulhm.cz/files/uploads/2020/10/Book-of-Abstracts_Forest-future-2020.pdf" target="_blank" >https://www.vulhm.cz/files/uploads/2020/10/Book-of-Abstracts_Forest-future-2020.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Utilization of SSR markers for identification of gene resources of small-leaved linden reproductive material

Popis výsledku v původním jazyce

Contribution published in an itnernational conference book of abstracts. Total genomic DNA was extracted by DNA Plant Mini Kit (QIAGEN) from young leaves taken from 377 sampled trees from 38 clones of the model seed orchard. Samples were screened using selected eight polymorphic nuclear microsatellite markers. The obtained data were analysed by statistical programs CERVUS, GenAlEx 6.503. There were detected 79 different alleles at 8 loci in the 377 lime individuals from seed orchard. The obtained results illustrate the utility of the microsatellite loci for assessing spatial patterns of genetic diversity and for individual genotypes identification. 93.6% of the sampled trees could be assigned to the clones represented in the model seed orchard. The identified genetic loci were verified as highly polymorphic and could be further used for clonal identification of lime trees.

Název v anglickém jazyce

Utilization of SSR markers for identification of gene resources of small-leaved linden reproductive material

Popis výsledku anglicky

Contribution published in an itnernational conference book of abstracts. Total genomic DNA was extracted by DNA Plant Mini Kit (QIAGEN) from young leaves taken from 377 sampled trees from 38 clones of the model seed orchard. Samples were screened using selected eight polymorphic nuclear microsatellite markers. The obtained data were analysed by statistical programs CERVUS, GenAlEx 6.503. There were detected 79 different alleles at 8 loci in the 377 lime individuals from seed orchard. The obtained results illustrate the utility of the microsatellite loci for assessing spatial patterns of genetic diversity and for individual genotypes identification. 93.6% of the sampled trees could be assigned to the clones represented in the model seed orchard. The identified genetic loci were verified as highly polymorphic and could be further used for clonal identification of lime trees.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40102 - Forestry

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů