Determining the Optimal Method for DNA Isolation from Fruit Jams

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F18%3A00004503" target="_blank" >RIV/00027006:_____/18:00004503 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17221/340/2017-CJFS" target="_blank" >http://dx.doi.org/10.17221/340/2017-CJFS</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/340/2017-CJFS" target="_blank" >10.17221/340/2017-CJFS</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determining the Optimal Method for DNA Isolation from Fruit Jams

Popis výsledku v původním jazyce

DNA extraction is a crucial step in PCR analysis especially when analysing food samples that can be degraded and can potentially contain PCR-inhibiting substances. In this study, we compared the suitability of three DNA extraction methods - two kits: DNeasy (R) Plant Mini Kit and NucleoSpin (R) Food, and the CTAB method - for DNA extraction from commercial fruit jams. Fourteen jams with different contents of fruit, sugar and other additives were extracted in triplicate using the above-mentioned methods directly and after a washing step. The concentration and optical density were analysed using UV spectrophotometry and the amplifiability of the obtained DNA was evaluated using a PCR assay targeting a sequence coding for chloroplast tRNA-Leu. Samples isolated using the NucleoSpin (R) Food kit contained non-amplifiable DNA in eight cases, and samples isolated using the CTAB method could not be quantified. The DNeasy (R) Plant Mini Kit thus proved to be the most suitable method, since well-amplifiable DNA was obtained for all the analysed samples.

Název v anglickém jazyce

Determining the Optimal Method for DNA Isolation from Fruit Jams

Popis výsledku anglicky

DNA extraction is a crucial step in PCR analysis especially when analysing food samples that can be degraded and can potentially contain PCR-inhibiting substances. In this study, we compared the suitability of three DNA extraction methods - two kits: DNeasy (R) Plant Mini Kit and NucleoSpin (R) Food, and the CTAB method - for DNA extraction from commercial fruit jams. Fourteen jams with different contents of fruit, sugar and other additives were extracted in triplicate using the above-mentioned methods directly and after a washing step. The concentration and optical density were analysed using UV spectrophotometry and the amplifiability of the obtained DNA was evaluated using a PCR assay targeting a sequence coding for chloroplast tRNA-Leu. Samples isolated using the NucleoSpin (R) Food kit contained non-amplifiable DNA in eight cases, and samples isolated using the CTAB method could not be quantified. The DNeasy (R) Plant Mini Kit thus proved to be the most suitable method, since well-amplifiable DNA was obtained for all the analysed samples.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

<a href="/cs/project/QJ1530272" target="_blank" >QJ1530272: Komplexní strategie pro efektivní odhalování falšování potravin v řetězci (prvo)výroba - spotřebitel</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Food Sciences

ISSN

1212-1800

e-ISSN

1805-9317

Svazek periodika

36

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

126-132

Kód UT WoS článku

000431537800003

EID výsledku v databázi Scopus

2-s2.0-85046690431