Thermal analysis for cryopreservation method improvement
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F18%3A00004964" target="_blank" >RIV/00027006:_____/18:00004964 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Thermal analysis for cryopreservation method improvement
Popis výsledku v původním jazyce
Changes in thermal characteristics of plant tissues are important for explants successful cryopreservation. These changes can be influenced by genotype of plant, preculture conditions of explants and osmotically active solution used. The effect of genotype was studied in three grapevine varieties with different sensitivity to dehydration. Different sensitivity of explants to dehydration influenced significantly their tolerance to cryopreservation. A significant effect of explants acclimation on their tolerance to dehydration before cryopreservation was detected in poplar explants. Low temperature treatment resulted in a significant increase in poplar explants tolerance to dehydration. In addition, the positive simultaneous effect of low temperature and osmotic treatment by 0.7M sucrose on explant tolerance to dehydration was demonstrated. Thermal analysis allowed control of the whole dehydration process, including optimal hydration level of explants before cryopreservation.
Název v anglickém jazyce
Thermal analysis for cryopreservation method improvement
Popis výsledku anglicky
Changes in thermal characteristics of plant tissues are important for explants successful cryopreservation. These changes can be influenced by genotype of plant, preculture conditions of explants and osmotically active solution used. The effect of genotype was studied in three grapevine varieties with different sensitivity to dehydration. Different sensitivity of explants to dehydration influenced significantly their tolerance to cryopreservation. A significant effect of explants acclimation on their tolerance to dehydration before cryopreservation was detected in poplar explants. Low temperature treatment resulted in a significant increase in poplar explants tolerance to dehydration. In addition, the positive simultaneous effect of low temperature and osmotic treatment by 0.7M sucrose on explant tolerance to dehydration was demonstrated. Thermal analysis allowed control of the whole dehydration process, including optimal hydration level of explants before cryopreservation.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
40401 - Agricultural biotechnology and food biotechnology
Návaznosti výsledku
Projekt
<a href="/cs/project/QJ1630301" target="_blank" >QJ1630301: Tvorba nových systémů biotechnologických opatření pro zachování a rozvoj biodiverzity zemědělských plodin a lesních dřevin</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů