The Cryopreservation of Dormant Buds of Raspberry (Rubus idaeus L.)
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F24%3A10177740" target="_blank" >RIV/00027006:_____/24:10177740 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The Cryopreservation of Dormant Buds of Raspberry (Rubus idaeus L.)
Popis výsledku v původním jazyce
Cryoconservation enables long-term preservation of genetic resources in vegetatively propagated plants. For frost-resistant woody plants, cryobanking dormant buds is a cost-effective option, allowing regeneration by grafting. This study aimed to develop a cryopreservation method for dormant raspberry buds (Rubus idaeus L.) using pre-dehydration and two-step freezing, suitable for in vitro regeneration. Dormant raspberry shoots (varieties Sanibelle and Willamette) were collected midwinter, and uninodal segments were dehydrated in a freezer. Optimal dehydration levels were determined by studying low-temperature phase transitions, water state, and water content. Buds were rehydrated in wet peat and regrown in perlite. Results showed that dehydration reduced melting and crystallization temperatures, with water activity below 0.9 preventing crystallization during cooling. This critical dehydration level enabled up to 74% viable buds after cryopreservation.
Název v anglickém jazyce
The Cryopreservation of Dormant Buds of Raspberry (Rubus idaeus L.)
Popis výsledku anglicky
Cryoconservation enables long-term preservation of genetic resources in vegetatively propagated plants. For frost-resistant woody plants, cryobanking dormant buds is a cost-effective option, allowing regeneration by grafting. This study aimed to develop a cryopreservation method for dormant raspberry buds (Rubus idaeus L.) using pre-dehydration and two-step freezing, suitable for in vitro regeneration. Dormant raspberry shoots (varieties Sanibelle and Willamette) were collected midwinter, and uninodal segments were dehydrated in a freezer. Optimal dehydration levels were determined by studying low-temperature phase transitions, water state, and water content. Buds were rehydrated in wet peat and regrown in perlite. Results showed that dehydration reduced melting and crystallization temperatures, with water activity below 0.9 preventing crystallization during cooling. This critical dehydration level enabled up to 74% viable buds after cryopreservation.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Návaznosti výsledku
Projekt
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Návaznosti
R - Projekt Ramcoveho programu EK
Ostatní
Rok uplatnění
2024
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů