What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10177942" target="_blank" >RIV/00027006:_____/25:10177942 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/25:43931814

Výsledek na webu

<a href="https://www.mdpi.com/1420-3049/30/4/924/pdf?version=1739785415" target="_blank" >https://www.mdpi.com/1420-3049/30/4/924/pdf?version=1739785415</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/molecules30040924" target="_blank" >10.3390/molecules30040924</a>

Jazyk výsledku

angličtina

Název v původním jazyce

What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer

Popis výsledku v původním jazyce

Pulsed electric field (PEF) has previously been recognized as a method of gentle food processing, and its use has been shown to be helpful in reducing the levels of toxigenic Fusarium micromycetes developed during malting. The aim of this study was to describe the effects of PEF on gene expression and metabolite production at the pre-finishing stage of barley malting by using a novel multi-omics data-driven approach. The study helps to uncover the processes occurring in the germinated grain and discusses the up-/downregulation of genes and metabolites in relation to fungal infection and/or PEF-induced abiotic stress. Among the factors upregulated by PEF and previously described as supportive against Fusarium diseases, we identified the increased expression of genes encoding vegetative gp1-like protein, which positively correlated with flavonoids, (methylsulfanyl)prop-2-enoates, triterpenoid glycosides, and indole alkaloids. On the other hand, some genes associated with barley resistance to fungal infection were also overexpressed in the untreated control (in particular, genes encoding ethylene response factor 3-like, putrescine hydroxycinnamoyltransferase 3-like, and dirigent protein 21-like). This study provides the first &apos;data-driven&apos; basic research results that contribute to the understanding of the role of PEF as an effective fungal decontamination strategy and allows the formulation of new hypotheses related to Fusarium pathogen crosstalk.

Název v anglickém jazyce

What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer

Popis výsledku anglicky

Pulsed electric field (PEF) has previously been recognized as a method of gentle food processing, and its use has been shown to be helpful in reducing the levels of toxigenic Fusarium micromycetes developed during malting. The aim of this study was to describe the effects of PEF on gene expression and metabolite production at the pre-finishing stage of barley malting by using a novel multi-omics data-driven approach. The study helps to uncover the processes occurring in the germinated grain and discusses the up-/downregulation of genes and metabolites in relation to fungal infection and/or PEF-induced abiotic stress. Among the factors upregulated by PEF and previously described as supportive against Fusarium diseases, we identified the increased expression of genes encoding vegetative gp1-like protein, which positively correlated with flavonoids, (methylsulfanyl)prop-2-enoates, triterpenoid glycosides, and indole alkaloids. On the other hand, some genes associated with barley resistance to fungal infection were also overexpressed in the untreated control (in particular, genes encoding ethylene response factor 3-like, putrescine hydroxycinnamoyltransferase 3-like, and dirigent protein 21-like). This study provides the first &apos;data-driven&apos; basic research results that contribute to the understanding of the role of PEF as an effective fungal decontamination strategy and allows the formulation of new hypotheses related to Fusarium pathogen crosstalk.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MOLECULES

ISSN

1420-3049

e-ISSN

1420-3049

Svazek periodika

30

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

15

Strana od-do

924

Kód UT WoS článku

001430091300001

EID výsledku v databázi Scopus

2-s2.0-85219174635