Dormant bud cryopreservation of Rubus idaeus L.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10178062" target="_blank" >RIV/00027006:_____/25:10178062 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.sciencedirect.com/journal/cryobiology/vol/119/suppl/C" target="_blank" >https://www.sciencedirect.com/journal/cryobiology/vol/119/suppl/C</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cryobiol.2025.105240" target="_blank" >10.1016/j.cryobiol.2025.105240</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Dormant bud cryopreservation of Rubus idaeus L.
Popis výsledku v původním jazyce
Cryopreservation of dormant buds offers a reliable and cost-effective method for the long-term preservation of plant genetic resources, particularly for woody plants like raspberry (Rubus idaeus L.). This study aimed to develop an optimized protocol for the cryopreservation of dormant raspberry buds by investigating the role of dehydration and freezing conditions in maintaining bud viability. Dormant canes of two raspberry varieties, 'Sanibelle' and 'Willamette', were collected and prepared by dehydrating single-node cane segments at -4 degrees C until the desired water content was achieved. The study focused on assessing the impact of dehydration on bud viability and the physical state of water within the buds, using differential scanning calorimetry to analyse thermal transitions during cooling and heating. The cryopreservation process followed a two-step freezing protocol, with the first step involving slow cooling to -30 degrees C, followed by rapid immersion in liquid nitrogen (-196 degrees C). After cryopreservation, buds were thawed either slowly at +4 degrees C or rapidly at +38 degrees C, with rehydration taking place over 14 days. The study found that the most effective preservation (74-86 % of buds) occurred when the buds were dehydrated to 22-23 % humidity and had a water activity of 0.83-0.85. Rapid thawing significantly improved bud survival, especially for moderately dehydrated buds (with water content 25-35 %), while recrystallization during slow thawing was associated with lower viability. The results highlight the complexity of cryopreservation protocols, where factors such as dehydration levels, cooling and thawing rates, and bud dormancy status play crucial roles in ensuring successful long-term storage.
Název v anglickém jazyce
Dormant bud cryopreservation of Rubus idaeus L.
Popis výsledku anglicky
Cryopreservation of dormant buds offers a reliable and cost-effective method for the long-term preservation of plant genetic resources, particularly for woody plants like raspberry (Rubus idaeus L.). This study aimed to develop an optimized protocol for the cryopreservation of dormant raspberry buds by investigating the role of dehydration and freezing conditions in maintaining bud viability. Dormant canes of two raspberry varieties, 'Sanibelle' and 'Willamette', were collected and prepared by dehydrating single-node cane segments at -4 degrees C until the desired water content was achieved. The study focused on assessing the impact of dehydration on bud viability and the physical state of water within the buds, using differential scanning calorimetry to analyse thermal transitions during cooling and heating. The cryopreservation process followed a two-step freezing protocol, with the first step involving slow cooling to -30 degrees C, followed by rapid immersion in liquid nitrogen (-196 degrees C). After cryopreservation, buds were thawed either slowly at +4 degrees C or rapidly at +38 degrees C, with rehydration taking place over 14 days. The study found that the most effective preservation (74-86 % of buds) occurred when the buds were dehydrated to 22-23 % humidity and had a water activity of 0.83-0.85. Rapid thawing significantly improved bud survival, especially for moderately dehydrated buds (with water content 25-35 %), while recrystallization during slow thawing was associated with lower viability. The results highlight the complexity of cryopreservation protocols, where factors such as dehydration levels, cooling and thawing rates, and bud dormancy status play crucial roles in ensuring successful long-term storage.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Návaznosti výsledku
Projekt
<a href="/cs/project/TO01000295" target="_blank" >TO01000295: Zdravé ovoce v měnících se klimatických podmínkách: vývoj nových biotechnologických postupů diagnostiky virů, studium vektorů, ozdravování a bezpečného uchovávání jahodníku a maliníku</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2025
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
CRYOBIOLOGY
ISSN
0011-2240
e-ISSN
—
Svazek periodika
119
Číslo periodika v rámci svazku
JUN 2025
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
10
Strana od-do
105240
Kód UT WoS článku
001458598800001
EID výsledku v databázi Scopus
2-s2.0-105001123280