ELISA Kit for Mustard Protein Determination: Interlaboratory study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F11%3A00000233" target="_blank" >RIV/00027022:_____/11:00000233 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

ELISA Kit for Mustard Protein Determination: Interlaboratory study

Popis výsledku v původním jazyce

A collaborative study in 12 laboratories was performed to prove the validation of the ELISA method developed for the quantitative mustard protein determination in foods. The ELISA kit used for this study is based on rabbit polyclonal antibody. This kit did not produce any false positive results or cross-reactivity in in-house validation with a broad range of food matrices range with no detectable mustard protein . All participants obtained the Mustard ELISA kit with standard operational procedures, thelist of samples, samples and a protocol for recording test results. The study included 15 food samples and 2 spiked samples. The statistical tests (Cochran, Dixon and Mandel) and analysis of variance (ANOVA) were used for the evaluation of the collaborative study results. Repeatability and reproducibility limits as well as a limit of quantification (LOQ, 1.8 mg mustard proteins/kg) and a limit of detection (LOD, 0.5 mg mustard proteins/kg) for the kit were calculated.

Název v anglickém jazyce

ELISA Kit for Mustard Protein Determination: Interlaboratory study

Popis výsledku anglicky

A collaborative study in 12 laboratories was performed to prove the validation of the ELISA method developed for the quantitative mustard protein determination in foods. The ELISA kit used for this study is based on rabbit polyclonal antibody. This kit did not produce any false positive results or cross-reactivity in in-house validation with a broad range of food matrices range with no detectable mustard protein . All participants obtained the Mustard ELISA kit with standard operational procedures, thelist of samples, samples and a protocol for recording test results. The study included 15 food samples and 2 spiked samples. The statistical tests (Cochran, Dixon and Mandel) and analysis of variance (ANOVA) were used for the evaluation of the collaborative study results. Repeatability and reproducibility limits as well as a limit of quantification (LOQ, 1.8 mg mustard proteins/kg) and a limit of detection (LOD, 0.5 mg mustard proteins/kg) for the kit were calculated.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of AOAC International

ISSN

1060-3271

e-ISSN

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Svazek periodika

94

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

605-610

Kód UT WoS článku

000289544500029

EID výsledku v databázi Scopus

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