Prevalence, characterization and antimicrobial susceptibility of Yersinia enterocolitica and other Yersinia species found in fruits and vegetables from the European Union

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000012" target="_blank" >RIV/00027162:_____/18:N0000012 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16270/18:43876422

Výsledek na webu

<a href="https://www.researchgate.net/publication/320092513_Prevalence_characterization_and_antimicrobial_susceptibility_of_Yersinia_enterocolitica_and_other_Yersinia_species_found_in_fruits_and_vegetables_from_the_European_Union" target="_blank" >https://www.researchgate.net/publication/320092513_Prevalence_characterization_and_antimicrobial_susceptibility_of_Yersinia_enterocolitica_and_other_Yersinia_species_found_in_fruits_and_vegetables_from_the_European_Union</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.foodcont.2017.08.038" target="_blank" >10.1016/j.foodcont.2017.08.038</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Prevalence, characterization and antimicrobial susceptibility of Yersinia enterocolitica and other Yersinia species found in fruits and vegetables from the European Union

Popis výsledku v původním jazyce

A total of 312 samples of fruits, vegetables and sprouts originating from the European Union states were examined for the presence of pathogenic and non-pathogenic Yersinia enterocolitica and other Yersinia spp. The samples were examined separately using both cultivation method and real-time quantitative PCR (qPCR) without pre-enrichment. The prevalence of Yersinia spp. in examined samples assessed by cultivation was 17.0% (53/312), with Y. enterocolitica and other Yersinia spp. comprising 5.8% (18/312) and 11.2% (35/312) of samples, respectively. The ail gene, which is associated with Y. enterocolitica pathogenicity, was detected only in one isolate (0.3%). Biotyping of isolates revealed only biotype 1A. Serotyping tests showed that four isolates belonged to serotype O:8 and one to serotype O:5. QPCR results confirmed the presence of Yersinia spp. and pathogenic Y. enterocolitica (carrying the ail gene) in 37 (11.9%) and 21 (6.7%) samples, respectively. Further, the susceptibility of Y enterocolitica and the other Yersinia spp. isolates was tested using the agar dilution method and the Vitek (R) 2 system. All samples were susceptible to ciprofloxacin, gentamicin, enrofloxacin, cefotaxime, imipenem, meropenem, tobramycin, ciprofloxacin, colistin, trimethoprim and trimethoprim/sulfamethoxazole. The most commonly found resistance was to ampicillin (100%), followed by erythromycin (98.1%), amoxicillin/clavulanic acid (75.5%) and streptomycin (39.6%). (C) 2017 Elsevier Ltd. All rights reserved.

Název v anglickém jazyce

Prevalence, characterization and antimicrobial susceptibility of Yersinia enterocolitica and other Yersinia species found in fruits and vegetables from the European Union

Popis výsledku anglicky

A total of 312 samples of fruits, vegetables and sprouts originating from the European Union states were examined for the presence of pathogenic and non-pathogenic Yersinia enterocolitica and other Yersinia spp. The samples were examined separately using both cultivation method and real-time quantitative PCR (qPCR) without pre-enrichment. The prevalence of Yersinia spp. in examined samples assessed by cultivation was 17.0% (53/312), with Y. enterocolitica and other Yersinia spp. comprising 5.8% (18/312) and 11.2% (35/312) of samples, respectively. The ail gene, which is associated with Y. enterocolitica pathogenicity, was detected only in one isolate (0.3%). Biotyping of isolates revealed only biotype 1A. Serotyping tests showed that four isolates belonged to serotype O:8 and one to serotype O:5. QPCR results confirmed the presence of Yersinia spp. and pathogenic Y. enterocolitica (carrying the ail gene) in 37 (11.9%) and 21 (6.7%) samples, respectively. Further, the susceptibility of Y enterocolitica and the other Yersinia spp. isolates was tested using the agar dilution method and the Vitek (R) 2 system. All samples were susceptible to ciprofloxacin, gentamicin, enrofloxacin, cefotaxime, imipenem, meropenem, tobramycin, ciprofloxacin, colistin, trimethoprim and trimethoprim/sulfamethoxazole. The most commonly found resistance was to ampicillin (100%), followed by erythromycin (98.1%), amoxicillin/clavulanic acid (75.5%) and streptomycin (39.6%). (C) 2017 Elsevier Ltd. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/LO1218" target="_blank" >LO1218: Zdravé zvíře jako zdroj zdravé potraviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Control

ISSN

0956-7135

e-ISSN

1873-7129

Svazek periodika

85

Číslo periodika v rámci svazku

March

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

161-167

Kód UT WoS článku

000419409000020

EID výsledku v databázi Scopus

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