Contamination of two food processing plants in the Czech Republic and link to human clinical cases

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000057" target="_blank" >RIV/00027162:_____/19:N0000057 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.cvent.com/events/international-symposium-on-problems-of-listeria-and-listeriosis-isopol-2019/event-summary-b4ec3590ab794cf687632a204a59c233.aspx?dvce=1" target="_blank" >http://www.cvent.com/events/international-symposium-on-problems-of-listeria-and-listeriosis-isopol-2019/event-summary-b4ec3590ab794cf687632a204a59c233.aspx?dvce=1</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Contamination of two food processing plants in the Czech Republic and link to human clinical cases

Popis výsledku v původním jazyce

International Symposium on Problems of Listeria and Listeriosis (ISOPOL), 24. – 27. 9. 2019, Toronto, Canada – poster. Listeria monocytogenes (Lm) is a cause of severe foodborne illnesses, the listeriosis. Persistence of Lm in food processing plants is an important factor in the contamination of food products and transmission of this foodborne pathogen to the human population. This study identified, from the food products and the processing environment, two different Lm contaminations in two food processing plants (A & B). Both Lm contaminations had two distinct indistinguishable AscI PFGE profiles divergent by three bands. Eight isolates from plant A, over 2004 – 2015 and 11 isolates from plant B over 2004 – 2016 were sequenced together with indistinguishable AscI PFGE profiles human clinical strains, reported in the Czech Republic up to 2018. For plant strains, the threshold to consider putatively persistent (PP) was set at ≤ 35 SNPs difference pairwise. For human clinical strains, the epidemic link threshold between human and food was ≤ 7 allelic differences (AD) by core genome MLST. For both plants the strains were ST204. One PP strain lineage was detected in plant A (7 of the 8 strains) and another from plant B (10 of the 11 strains) both plant lineages were separated by ≤35-60 SNPs, except for two strains distant by 34 SNPs. Both were mainly isolated from the equipment of the plant. One human clinical strain isolated in 2011, had an epidemiological link with the plant A PP strain (4 AD). Two human clinical strains isolated in 2008 and 2011 had an epidemiological link with two plant B PP strains (2-6 AD). Although ST204 is observed in various environment and in food products, it is surprising to observe ST204 phylogenetically distant PP strains in distant plants (350 km), using raw material from different origins one from various EU countries, the other from its own production. This study WGS typing of Lm strains contaminating plants enabled to solve public health investigation. The results obtained indicated a particular affinity of ST204 to the plant environment. It provided an insight on the contamination structure in the plants. Further to this study, the equipment identified as contaminated by PP strains were sanitized. However, after production started contamination came back.

Název v anglickém jazyce

Contamination of two food processing plants in the Czech Republic and link to human clinical cases

Popis výsledku anglicky

International Symposium on Problems of Listeria and Listeriosis (ISOPOL), 24. – 27. 9. 2019, Toronto, Canada – poster. Listeria monocytogenes (Lm) is a cause of severe foodborne illnesses, the listeriosis. Persistence of Lm in food processing plants is an important factor in the contamination of food products and transmission of this foodborne pathogen to the human population. This study identified, from the food products and the processing environment, two different Lm contaminations in two food processing plants (A & B). Both Lm contaminations had two distinct indistinguishable AscI PFGE profiles divergent by three bands. Eight isolates from plant A, over 2004 – 2015 and 11 isolates from plant B over 2004 – 2016 were sequenced together with indistinguishable AscI PFGE profiles human clinical strains, reported in the Czech Republic up to 2018. For plant strains, the threshold to consider putatively persistent (PP) was set at ≤ 35 SNPs difference pairwise. For human clinical strains, the epidemic link threshold between human and food was ≤ 7 allelic differences (AD) by core genome MLST. For both plants the strains were ST204. One PP strain lineage was detected in plant A (7 of the 8 strains) and another from plant B (10 of the 11 strains) both plant lineages were separated by ≤35-60 SNPs, except for two strains distant by 34 SNPs. Both were mainly isolated from the equipment of the plant. One human clinical strain isolated in 2011, had an epidemiological link with the plant A PP strain (4 AD). Two human clinical strains isolated in 2008 and 2011 had an epidemiological link with two plant B PP strains (2-6 AD). Although ST204 is observed in various environment and in food products, it is surprising to observe ST204 phylogenetically distant PP strains in distant plants (350 km), using raw material from different origins one from various EU countries, the other from its own production. This study WGS typing of Lm strains contaminating plants enabled to solve public health investigation. The results obtained indicated a particular affinity of ST204 to the plant environment. It provided an insight on the contamination structure in the plants. Further to this study, the equipment identified as contaminated by PP strains were sanitized. However, after production started contamination came back.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/QK1910121" target="_blank" >QK1910121: Perzistence vybraných původců alimentárních onemocnění, hygienických indikátorů a možnosti jejich eliminace z prostředí potravinářských podniků</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů