European-wide study reveals high prevalence of hypervirulent Listeria monocytogenes clones in farmed ruminants and their environment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000146" target="_blank" >RIV/00027162:_____/19:N0000146 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.cvent.com/events/international-symposium-on-problems-of-listeria-and-listeriosis-isopol-2019/event-summary-b4ec3590ab794cf687632a204a59c233.aspx?dvce=1" target="_blank" >http://www.cvent.com/events/international-symposium-on-problems-of-listeria-and-listeriosis-isopol-2019/event-summary-b4ec3590ab794cf687632a204a59c233.aspx?dvce=1</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

European-wide study reveals high prevalence of hypervirulent Listeria monocytogenes clones in farmed ruminants and their environment

Popis výsledku v původním jazyce

International Symposium on Problems of Listeria and Listeriosis (ISOPOL), 24. – 27. 9. 2019, Toronto, Canada – poster. Listeria monocytogenes (Lm) frequently causes neurolisteriosis and abortion in farmed ruminants, leading to economic losses in the livestock industry. Moreover, Lm is a common contaminant of raw milk and food production units. Farmed ruminants, in particular cattle, are considered a reservoir of Lm strains responsible for human infections through the contamination of dairy products. Previous studies indicate that hypervirulent clonal complexes (CCs) such as CC1 and CC4 are associated with human and ruminant infection and additionally prevail in dairy products. These CCs are also found in the farm environment. In this European-wide study, we assess the distribution of CCs in a panel of 1014 Lm isolates from farmed ruminants and their environment in 13 different countries. So far, we analysed the CC diversity of 669, most of them originating from CH, FR and SI. Isolates were classified into two groups: 1) clinical (isolates from diseased animals, n=425) and 2) non-clinical (isolates from faeces of apparently healthy ruminants, n=100 or the farm environment, n=144). In total, 59 CCs were identified. CC1, 4, 37 and 6 were the most frequent CCs both in clinical and non-clinical isolates. However, while prevalences in clinical and non-clinical isolates were similar for CC4 (12.6% versus 7.7%), CC37 (7.0% versus 7.2%) and CC6 (5.4% versus 7.2%), CC1 was significantly associated with clinical origin (38.8% versus 10.2%, p-value<0.0001). CCs that were significantly associated with non-clinical origin included CC5 (0.5% versus 5.5%), CC14 (2.1% versus 5.5%) and CC29 (0.5% versus 4.7%, p-value<0.0001). Within non-clinical isolates, CC8 was significantly associated with the farm environment (p-value<0.025), while the remaining CCs were distributed uniformly between farm environment and the other non-clinical isolates. The obtained results show that farmed ruminants and their environment harbour diverse CCs of Lm. However, hypervirulent clones associated with human infection (in particular CC1) clearly belong to the most frequent clones in farmed ruminants and the farm environment at the European scale further supporting their role as a potential source for hypervirulent Lm clones. Consideration of these observations may help to improve surveillance and control of listeriosis.

Název v anglickém jazyce

European-wide study reveals high prevalence of hypervirulent Listeria monocytogenes clones in farmed ruminants and their environment

Popis výsledku anglicky

International Symposium on Problems of Listeria and Listeriosis (ISOPOL), 24. – 27. 9. 2019, Toronto, Canada – poster. Listeria monocytogenes (Lm) frequently causes neurolisteriosis and abortion in farmed ruminants, leading to economic losses in the livestock industry. Moreover, Lm is a common contaminant of raw milk and food production units. Farmed ruminants, in particular cattle, are considered a reservoir of Lm strains responsible for human infections through the contamination of dairy products. Previous studies indicate that hypervirulent clonal complexes (CCs) such as CC1 and CC4 are associated with human and ruminant infection and additionally prevail in dairy products. These CCs are also found in the farm environment. In this European-wide study, we assess the distribution of CCs in a panel of 1014 Lm isolates from farmed ruminants and their environment in 13 different countries. So far, we analysed the CC diversity of 669, most of them originating from CH, FR and SI. Isolates were classified into two groups: 1) clinical (isolates from diseased animals, n=425) and 2) non-clinical (isolates from faeces of apparently healthy ruminants, n=100 or the farm environment, n=144). In total, 59 CCs were identified. CC1, 4, 37 and 6 were the most frequent CCs both in clinical and non-clinical isolates. However, while prevalences in clinical and non-clinical isolates were similar for CC4 (12.6% versus 7.7%), CC37 (7.0% versus 7.2%) and CC6 (5.4% versus 7.2%), CC1 was significantly associated with clinical origin (38.8% versus 10.2%, p-value<0.0001). CCs that were significantly associated with non-clinical origin included CC5 (0.5% versus 5.5%), CC14 (2.1% versus 5.5%) and CC29 (0.5% versus 4.7%, p-value<0.0001). Within non-clinical isolates, CC8 was significantly associated with the farm environment (p-value<0.025), while the remaining CCs were distributed uniformly between farm environment and the other non-clinical isolates. The obtained results show that farmed ruminants and their environment harbour diverse CCs of Lm. However, hypervirulent clones associated with human infection (in particular CC1) clearly belong to the most frequent clones in farmed ruminants and the farm environment at the European scale further supporting their role as a potential source for hypervirulent Lm clones. Consideration of these observations may help to improve surveillance and control of listeriosis.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů