Raw bovine milk as a reservoir of yeast with virulence factors and decreased susceptibility to antifungal agents

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000168" target="_blank" >RIV/00027162:_____/21:N0000168 - isvavai.cz</a>

Výsledek na webu

<a href="https://academic.oup.com/mmy/article-abstract/59/10/1032/6309310?redirectedFrom=fulltext" target="_blank" >https://academic.oup.com/mmy/article-abstract/59/10/1032/6309310?redirectedFrom=fulltext</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/mmy/myab036" target="_blank" >10.1093/mmy/myab036</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Raw bovine milk as a reservoir of yeast with virulence factors and decreased susceptibility to antifungal agents

Popis výsledku v původním jazyce

In recent years, increased rates of yeast infections in humans and animals are recognized worldwide. Considering that animals can represent a source of yeast infections for humans, knowing the antifungal susceptibility profile of yeast isolates from milk and evaluating their pathogenic potential would be of great medical importance. Therefore, the aim of this survey was to study yeast diversity in milk samples, determine hemolytic and phospholipase activities of isolates and establish minimal inhibition concentration (MIC) for fluconazole, voriconazole and flucytosine, using microdilution method. Out of 66 yeast isolates from raw milk samples of 910 subclinically infected cows, 26 different yeast species were determined based on sequencing of the D1/D2 region and ITS region. Among them, Pichia kudriavzevii (9; Candida krusei), Kluyveromyces marxianus (8; Candida kefyr) and Debaryomyces hansenii (8; Candida famata) were the most commonly identified. Hemolysin and phospholipase activity was observed in more than half percent of isolates, separately. The elevated MIC for fluconazole above 4 mg/ml was determined in 16 isolates of 11 species except for intrinsically resistant isolates of P. kudriavzevii. The findings of this study demonstrate that yeast isolates from raw milk have the potential to express virulence attributes such as hemolysin and phospholipase and additionally, some of these strains showed elevated MIC to fluconazole or to flucytosin.

Název v anglickém jazyce

Raw bovine milk as a reservoir of yeast with virulence factors and decreased susceptibility to antifungal agents

Popis výsledku anglicky

In recent years, increased rates of yeast infections in humans and animals are recognized worldwide. Considering that animals can represent a source of yeast infections for humans, knowing the antifungal susceptibility profile of yeast isolates from milk and evaluating their pathogenic potential would be of great medical importance. Therefore, the aim of this survey was to study yeast diversity in milk samples, determine hemolytic and phospholipase activities of isolates and establish minimal inhibition concentration (MIC) for fluconazole, voriconazole and flucytosine, using microdilution method. Out of 66 yeast isolates from raw milk samples of 910 subclinically infected cows, 26 different yeast species were determined based on sequencing of the D1/D2 region and ITS region. Among them, Pichia kudriavzevii (9; Candida krusei), Kluyveromyces marxianus (8; Candida kefyr) and Debaryomyces hansenii (8; Candida famata) were the most commonly identified. Hemolysin and phospholipase activity was observed in more than half percent of isolates, separately. The elevated MIC for fluconazole above 4 mg/ml was determined in 16 isolates of 11 species except for intrinsically resistant isolates of P. kudriavzevii. The findings of this study demonstrate that yeast isolates from raw milk have the potential to express virulence attributes such as hemolysin and phospholipase and additionally, some of these strains showed elevated MIC to fluconazole or to flucytosin.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40200 - Animal and Dairy science

Projekt

<a href="/cs/project/QK1910092" target="_blank" >QK1910092: Nebakteriální původci mastitid a jejich vliv na kvalitu a technologické vlastnosti mléka</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Medical Mycology

ISSN

1369-3786

e-ISSN

1460-2709

Svazek periodika

59

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

1032–1040

Kód UT WoS článku

000709571800011

EID výsledku v databázi Scopus

2-s2.0-85117388473