Enrichment of circulating trophoblasts from maternal blood using filtration-based Metacell(R) technology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F22%3A43923718" target="_blank" >RIV/00064173:_____/22:43923718 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1371/journal.pone.0271226" target="_blank" >https://doi.org/10.1371/journal.pone.0271226</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0271226" target="_blank" >10.1371/journal.pone.0271226</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enrichment of circulating trophoblasts from maternal blood using filtration-based Metacell(R) technology

Popis výsledku v původním jazyce

In a cell-based non-invasive prenatal test (cbNIPT), intact circulating trophoblasts (CTs) are isolated from maternal blood for subsequent genetic analysis. Enrichment of these CTs from maternal blood is the most challenging step in the cbNIPT workflow. This study aims to assess the suitability of the filtration-based Metacell(R) technology to enrich CTs from maternal blood at week 10 to 13 of gestation. The Metacell(R) technology is a novel size-based enrichment technology that combines blood filtration through 8 μm pores with an in vitro culture method. Three protocols were evaluated. First, 8 mL or 16 mL of maternal blood was filtered and subsequently cultured in vitro on the separation membrane for 3 days in RPMI 1640. In addition, 16 mL of maternal blood was filtered, and immediately processed without further culturing. Y-chromosome-specific qPCR or STR analysis was performed to evaluate the enrichment of CTs. A total of 44 samples from pregnant women, out of which 26 were carrying a male fetus, were processed. Although five enriched male fetus samples show detectable male DNA quantities, it cannot be excluded that the obtained positive signal is caused by cell-free fetal DNA sticking to the Metacell(R) separation membrane. In conclusion, the Metacell(R) technology, tested as described, is not suitable for consistent enrichment of CTs.

Název v anglickém jazyce

Enrichment of circulating trophoblasts from maternal blood using filtration-based Metacell(R) technology

Popis výsledku anglicky

In a cell-based non-invasive prenatal test (cbNIPT), intact circulating trophoblasts (CTs) are isolated from maternal blood for subsequent genetic analysis. Enrichment of these CTs from maternal blood is the most challenging step in the cbNIPT workflow. This study aims to assess the suitability of the filtration-based Metacell(R) technology to enrich CTs from maternal blood at week 10 to 13 of gestation. The Metacell(R) technology is a novel size-based enrichment technology that combines blood filtration through 8 μm pores with an in vitro culture method. Three protocols were evaluated. First, 8 mL or 16 mL of maternal blood was filtered and subsequently cultured in vitro on the separation membrane for 3 days in RPMI 1640. In addition, 16 mL of maternal blood was filtered, and immediately processed without further culturing. Y-chromosome-specific qPCR or STR analysis was performed to evaluate the enrichment of CTs. A total of 44 samples from pregnant women, out of which 26 were carrying a male fetus, were processed. Although five enriched male fetus samples show detectable male DNA quantities, it cannot be excluded that the obtained positive signal is caused by cell-free fetal DNA sticking to the Metacell(R) separation membrane. In conclusion, the Metacell(R) technology, tested as described, is not suitable for consistent enrichment of CTs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30101 - Human genetics

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS One

ISSN

1932-6203

e-ISSN

1932-6203

Svazek periodika

17

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

"e0271226"

Kód UT WoS článku

000922616700001

EID výsledku v databázi Scopus

2-s2.0-85134166331