Glucocerebrosidase gene has an alternative upstream promoter, which has features and expression characteristic of housekeeping genes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A9903" target="_blank" >RIV/00216208:11110/11:9903 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023001:_____/11:00002460 RIV/00064165:_____/11:9903

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.bcmd.2010.12.011" target="_blank" >http://dx.doi.org/10.1016/j.bcmd.2010.12.011</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Glucocerebrosidase gene has an alternative upstream promoter, which has features and expression characteristic of housekeeping genes

Popis výsledku v původním jazyce

Database searches have shown that a part of glucocerebrosidase (GBA) transcripts may originate at an alternative upstream promoter (P2) located 2.6 kb upstream of the known (P1) GBA promoter. Luciferase assays confirmed promoter activity of both sites inHepG2 cells: the P1 construct exhibited higher activity of luciferase than the P2. Serial 5 deletions of P2 led to changes in reporter activity. Three P2 transcription initiation sites were found by 5 RACE at positions 347, 380 and 413 bp upstream of the +1 ATG. The expression stability of transcripts from P2, P1 was studied in 20 human tissues and was higher than that of GAPDH and ACTB, which are commonly used as reference housekeeping genes. The P2 contains an unmethylated CpG island, multiple Sp-1 consensus binding sites and, unlike P1, does not contain a TATA box, features all common to the majority of housekeeping gene promoters.

Název v anglickém jazyce

Glucocerebrosidase gene has an alternative upstream promoter, which has features and expression characteristic of housekeeping genes

Popis výsledku anglicky

Database searches have shown that a part of glucocerebrosidase (GBA) transcripts may originate at an alternative upstream promoter (P2) located 2.6 kb upstream of the known (P1) GBA promoter. Luciferase assays confirmed promoter activity of both sites inHepG2 cells: the P1 construct exhibited higher activity of luciferase than the P2. Serial 5 deletions of P2 led to changes in reporter activity. Three P2 transcription initiation sites were found by 5 RACE at positions 347, 380 and 413 bp upstream of the +1 ATG. The expression stability of transcripts from P2, P1 was studied in 20 human tissues and was higher than that of GAPDH and ACTB, which are commonly used as reference housekeeping genes. The P2 contains an unmethylated CpG island, multiple Sp-1 consensus binding sites and, unlike P1, does not contain a TATA box, features all common to the majority of housekeeping gene promoters.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Blood Cells Molecules and Diseases

ISSN

1079-9796

e-ISSN

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Svazek periodika

46

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

239-245

Kód UT WoS článku

000288574700010

EID výsledku v databázi Scopus

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