Allergenicity of main celery allergen rApi g1 and high-pressure treatment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F09%3A00002247" target="_blank" >RIV/00216208:11120/09:00002247 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064173:_____/09:00002247

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Allergenicity of main celery allergen rApi g1 and high-pressure treatment

Popis výsledku v původním jazyce

The structural changes and allergenicity of recombinant main celery allergen rApi g1 caused by the high pressure were studied. We have treated the buffer solutions of rApi g1 by high pressure at 500MPa for pressure holding times 10 and 20min and holdingtime temperatures 30 degrees C, 40 degrees C and 50 degrees C. The structural changes were studied by circular dichroismus (CD) spectra. The allergenic reaction of the rApi g1 was tested by Western blot analysis. The greatest changes of the structure were found at samples treated by 500MPa at 50 degrees C. The samples treated at this temperature at pressure levels 400, 450 and 500MPa and held for 10 and 20min showed that protein structure changes are positively correlated with pressure. Western blot analysis evidenced that a pressure of 500MPa held for 10-20min at temperatures 30-50 degrees C did not change the allergenicity of the rApi g1 protein when compared with the untreated sample.

Název v anglickém jazyce

Allergenicity of main celery allergen rApi g1 and high-pressure treatment

Popis výsledku anglicky

The structural changes and allergenicity of recombinant main celery allergen rApi g1 caused by the high pressure were studied. We have treated the buffer solutions of rApi g1 by high pressure at 500MPa for pressure holding times 10 and 20min and holdingtime temperatures 30 degrees C, 40 degrees C and 50 degrees C. The structural changes were studied by circular dichroismus (CD) spectra. The allergenic reaction of the rApi g1 was tested by Western blot analysis. The greatest changes of the structure were found at samples treated by 500MPa at 50 degrees C. The samples treated at this temperature at pressure levels 400, 450 and 500MPa and held for 10 and 20min showed that protein structure changes are positively correlated with pressure. Western blot analysis evidenced that a pressure of 500MPa held for 10-20min at temperatures 30-50 degrees C did not change the allergenicity of the rApi g1 protein when compared with the untreated sample.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

High Pressure Research

ISSN

0895-7959

e-ISSN

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Svazek periodika

29

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

19

Strana od-do

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Kód UT WoS článku

000274675700042

EID výsledku v databázi Scopus

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