Allergenicity of main birch allergen rBet v1 and high-pressure treatment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F09%3A00002248" target="_blank" >RIV/00216208:11120/09:00002248 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064173:_____/09:00002248

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Allergenicity of main birch allergen rBet v1 and high-pressure treatment

Popis výsledku v původním jazyce

The aim of our study was to determine the influence of high-pressure treatment on the structure and allergenicity of recombinant rBet v1, the main birch allergen and birch pollen extract. We treated the buffered solutions of rBet v1 and birch pollen extract with high pressure (450-550MPa) for a period of 10 min at temperatures between 30 and 50 C. The structural changes in rBet v1 were studied using circular dichroism spectra. The greatest changes in the rBet v1 structure were found in the samples treated at 450MPa at 30 C. The samples treated at 500 and 550MPa at 30 C (10 min) did not show any structural changes. The allergenic reaction to rBet v1 and the birch pollen extract was tested using Western blot analysis. Pressures of 450-550MPa (10 min) attemperatures of 30, 40 and 50 C did not change the allergenicity of the rBet v1 protein or the birch pollen extract when compared with untreated samples.

Název v anglickém jazyce

Allergenicity of main birch allergen rBet v1 and high-pressure treatment

Popis výsledku anglicky

The aim of our study was to determine the influence of high-pressure treatment on the structure and allergenicity of recombinant rBet v1, the main birch allergen and birch pollen extract. We treated the buffered solutions of rBet v1 and birch pollen extract with high pressure (450-550MPa) for a period of 10 min at temperatures between 30 and 50 C. The structural changes in rBet v1 were studied using circular dichroism spectra. The greatest changes in the rBet v1 structure were found in the samples treated at 450MPa at 30 C. The samples treated at 500 and 550MPa at 30 C (10 min) did not show any structural changes. The allergenic reaction to rBet v1 and the birch pollen extract was tested using Western blot analysis. Pressures of 450-550MPa (10 min) attemperatures of 30, 40 and 50 C did not change the allergenicity of the rBet v1 protein or the birch pollen extract when compared with untreated samples.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

High Pressure Research

ISSN

0895-7959

e-ISSN

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Svazek periodika

29

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

000274675700041

EID výsledku v databázi Scopus

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