Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F09%3A5515" target="_blank" >RIV/00216208:11130/09:5515 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/09:5515

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction

Popis výsledku v původním jazyce

We investigated the effects of two surface active agents (sodium dodecyl sulfate, SDS, and polyoxyethylene sorbitol, Tween-20), ionic strength and reduced glutathione (GSH) on MT signals measured by adsorptive transfer stripping differential pulse voltammetry Brdicka reaction. If we compared the GSH and MT signals at the accumulation time of 120 s, the MT signal was more than two hundred times higher than the GSH one. The influence of Tween-20 was investigated in a broad concentration range (from 10(-8)to 10(-11) %, v/v). We found that the catalytic Cat2 signal decreased for more than 60% with increasing Tween-20 content. In the case of using SDS (10(-3) - 10(-6) M), the increase of Cat2 peak in the presence of the highest SDS concentration was determined. The height of Cat2 peak enhanced with increasing ionic strength mimicking by NaCl concentration. Based on the enhancement of the Cat2 peak we determined the ionic strength of fibroblasts sample and recalculated the concentration of

Název v anglickém jazyce

Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction

Popis výsledku anglicky

We investigated the effects of two surface active agents (sodium dodecyl sulfate, SDS, and polyoxyethylene sorbitol, Tween-20), ionic strength and reduced glutathione (GSH) on MT signals measured by adsorptive transfer stripping differential pulse voltammetry Brdicka reaction. If we compared the GSH and MT signals at the accumulation time of 120 s, the MT signal was more than two hundred times higher than the GSH one. The influence of Tween-20 was investigated in a broad concentration range (from 10(-8)to 10(-11) %, v/v). We found that the catalytic Cat2 signal decreased for more than 60% with increasing Tween-20 content. In the case of using SDS (10(-3) - 10(-6) M), the increase of Cat2 peak in the presence of the highest SDS concentration was determined. The height of Cat2 peak enhanced with increasing ionic strength mimicking by NaCl concentration. Based on the enhancement of the Cat2 peak we determined the ionic strength of fibroblasts sample and recalculated the concentration of

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/IAA401990701" target="_blank" >IAA401990701: Studium vazby platinových cytostatik do struktury DNA; Vliv metalothioneinu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

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Svazek periodika

21

Číslo periodika v rámci svazku

3-5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

000264063500057

EID výsledku v databázi Scopus

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