A simple, time-saving, microwave-assisted periodic acid-Schiff's staining of glycoproteins on 1D electrophoretic gels

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F17%3A10365442" target="_blank" >RIV/00216208:11140/17:10365442 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/elps.201700189/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/elps.201700189/epdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201700189" target="_blank" >10.1002/elps.201700189</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A simple, time-saving, microwave-assisted periodic acid-Schiff's staining of glycoproteins on 1D electrophoretic gels

Popis výsledku v původním jazyce

We introduce an optimized periodic acid-Schiff&apos;s staining of glycoproteins on 1D electrophoretic gels. Thanks to heating in a household microwave oven the protocol of standard periodic acid-Schiff&apos;s staining has been accelerated from 6 h to below 10 min employing standard chemistry. At the same time, we show that the microwave-assisted glycoprotein staining is at least as sensitive as the conventional approach. All glycoproteins stained by the microwave-accelerated procedure were successfully identified using MALDI TOF/TOF mass spectrometry. The ensuing reduction in gel staining time and simplification of the staining protocol should significantly increase laboratory throughput when glycoprotein detection on electrophoretic gels is required in large numbers.

Název v anglickém jazyce

A simple, time-saving, microwave-assisted periodic acid-Schiff's staining of glycoproteins on 1D electrophoretic gels

Popis výsledku anglicky

We introduce an optimized periodic acid-Schiff&apos;s staining of glycoproteins on 1D electrophoretic gels. Thanks to heating in a household microwave oven the protocol of standard periodic acid-Schiff&apos;s staining has been accelerated from 6 h to below 10 min employing standard chemistry. At the same time, we show that the microwave-assisted glycoprotein staining is at least as sensitive as the conventional approach. All glycoproteins stained by the microwave-accelerated procedure were successfully identified using MALDI TOF/TOF mass spectrometry. The ensuing reduction in gel staining time and simplification of the staining protocol should significantly increase laboratory throughput when glycoprotein detection on electrophoretic gels is required in large numbers.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LO1503" target="_blank" >LO1503: BIOMEDIC</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Svazek periodika

38

Číslo periodika v rámci svazku

24

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

4

Strana od-do

3100-3103

Kód UT WoS článku

000417755800004

EID výsledku v databázi Scopus

2-s2.0-85037550015