Development of a Gradient HPLC Method for the Simultaneous Determination of Sotalol and Sorbate in Oral Liquid Preparations Using Solid Core Stationary Phase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F15%3A10294607" target="_blank" >RIV/00216208:11160/15:10294607 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/15:10294607

Výsledek na webu

<a href="http://www.hindawi.com/journals/jamc/2015/806736/" target="_blank" >http://www.hindawi.com/journals/jamc/2015/806736/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2015/806736" target="_blank" >10.1155/2015/806736</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of a Gradient HPLC Method for the Simultaneous Determination of Sotalol and Sorbate in Oral Liquid Preparations Using Solid Core Stationary Phase

Popis výsledku v původním jazyce

A selective and sensitive gradient HPLC-UV method for quantification of sotalol hydrochloride and potassium sorbate in five types of oral liquid preparations was developed and fully validated. The separation of an active substance sotalol hydrochloride,potassium sorbate (antimicrobial agent), and other substances (for taste and smell correction, etc.) was performed using an Ascentis Express C18 (100 x 4.6 mm, particles 2.7 mu m) solid core HPLC column. Linear gradient elution mode with a flow rate of 1.3 mL min(-1) was used, and the injection volume was 5 mu L. The UV/Vis absorbance detector was set to a wavelength of 237 nm, and the column oven was conditioned at 25 degrees C. A sodium dihydrogen phosphate dihydrate solution (pH 2.5; 17.7 mM) was used as the mobile phase buffer. The total analysis time was 4.5 min (+ 2.5 min for reequilibration). The method was successfully employed in a stability evaluation of the developed formulations, which are now already being used in the thera

Název v anglickém jazyce

Development of a Gradient HPLC Method for the Simultaneous Determination of Sotalol and Sorbate in Oral Liquid Preparations Using Solid Core Stationary Phase

Popis výsledku anglicky

A selective and sensitive gradient HPLC-UV method for quantification of sotalol hydrochloride and potassium sorbate in five types of oral liquid preparations was developed and fully validated. The separation of an active substance sotalol hydrochloride,potassium sorbate (antimicrobial agent), and other substances (for taste and smell correction, etc.) was performed using an Ascentis Express C18 (100 x 4.6 mm, particles 2.7 mu m) solid core HPLC column. Linear gradient elution mode with a flow rate of 1.3 mL min(-1) was used, and the injection volume was 5 mu L. The UV/Vis absorbance detector was set to a wavelength of 237 nm, and the column oven was conditioned at 25 degrees C. A sodium dihydrogen phosphate dihydrate solution (pH 2.5; 17.7 mM) was used as the mobile phase buffer. The total analysis time was 4.5 min (+ 2.5 min for reequilibration). The method was successfully employed in a stability evaluation of the developed formulations, which are now already being used in the thera

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.20.0235" target="_blank" >EE2.3.20.0235: Vybudování výzkumného týmu experimentální a aplikované biofarmacie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Analytical Methods in Chemistry

ISSN

2090-8865

e-ISSN

—

Svazek periodika

2015

Číslo periodika v rámci svazku

March

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

000352380300001

EID výsledku v databázi Scopus

2-s2.0-84926637040