Hematoxylin assay of cupric chelation can give false positive results

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F19%3A10399578" target="_blank" >RIV/00216208:11160/19:10399578 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=CUMhbu5b9n" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=CUMhbu5b9n</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jtemb.2018.10.022" target="_blank" >10.1016/j.jtemb.2018.10.022</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Hematoxylin assay of cupric chelation can give false positive results

Popis výsledku v původním jazyce

Some compounds without apparent chelation sites have been shown to chelate cupric ions using the hematoxylin assay. Four different known reducing agents (hydroxylamine, vitamin C, trolox, reduced glutathione /GSH/) were selected for the study together with oxidized glutathione (GSSG). All tested compounds behaved as cupric chelators in the spectrophotometric mildly competitive hematoxylin assay. In-depth analysis however showed that only GSH and GSSG were able to form complexes with both cupric and cuprous ions and only GSSG partly retained copper in its complexes in the more competitive bathocuproine assay. All tested compounds with the exception of GSSG reduced Cu2+ ions. Compounds reducing copper such as antioxidants can give false positive results in the hematoxylin-screening assay.

Název v anglickém jazyce

Hematoxylin assay of cupric chelation can give false positive results

Popis výsledku anglicky

Some compounds without apparent chelation sites have been shown to chelate cupric ions using the hematoxylin assay. Four different known reducing agents (hydroxylamine, vitamin C, trolox, reduced glutathione /GSH/) were selected for the study together with oxidized glutathione (GSSG). All tested compounds behaved as cupric chelators in the spectrophotometric mildly competitive hematoxylin assay. In-depth analysis however showed that only GSH and GSSG were able to form complexes with both cupric and cuprous ions and only GSSG partly retained copper in its complexes in the more competitive bathocuproine assay. All tested compounds with the exception of GSSG reduced Cu2+ ions. Compounds reducing copper such as antioxidants can give false positive results in the hematoxylin-screening assay.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/GA18-00121S" target="_blank" >GA18-00121S: Role opticky čistých flavonolignanů v kardiovaskulárních účincích silymarinu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Trace Elements in Medicine and Biology

ISSN

0946-672X

e-ISSN

—

Svazek periodika

52

Číslo periodika v rámci svazku

March

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

29-36

Kód UT WoS článku

000463958300006

EID výsledku v databázi Scopus

2-s2.0-85056737918