Study of DNA-ellipticine interaction by capillary electrophoresis with laser-induced fluorescence detection
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10126289" target="_blank" >RIV/00216208:11310/12:10126289 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62156489:43210/12:00189242 RIV/00216208:11130/12:8168 RIV/00216305:26620/12:PU99015 RIV/00064203:_____/12:8168
Výsledek na webu
<a href="http://dx.doi.org/10.1002/elps.201200056" target="_blank" >http://dx.doi.org/10.1002/elps.201200056</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201200056" target="_blank" >10.1002/elps.201200056</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Study of DNA-ellipticine interaction by capillary electrophoresis with laser-induced fluorescence detection
Popis výsledku v původním jazyce
Ellipticine (5,11-dimethyl-6H-pyrido[4,3-b]carbazole), an alkaloid isolated from Apocynaceae plants, exhibits an antitumor activity, which is exceptionally high against several specific types of tumors. Ellipticine is also interesting as an anticancer drug as it has limited side effects and lacks of hematological toxicity. Various methods to study intercalating activity of this drug have been developed. However, to our best knowledge, capillary electrophoresis (CE) as a technique combining high separation resolution with various detection options has never been used for these purposes. In this study, a novel separation method based on CE with laser-induced fluorescence (CE-LIF) detection has been developed for the determination of ellipticine and for the monitoring of ellipticineDNA interaction. Sodium acetate (50 mM, pH 4.5) was used as a background electrolyte and LIF detection at ?ex = 488 nm. The limit of detection for ellipticine was determined to be 5 x 10(-8) M. A total of 20% d
Název v anglickém jazyce
Study of DNA-ellipticine interaction by capillary electrophoresis with laser-induced fluorescence detection
Popis výsledku anglicky
Ellipticine (5,11-dimethyl-6H-pyrido[4,3-b]carbazole), an alkaloid isolated from Apocynaceae plants, exhibits an antitumor activity, which is exceptionally high against several specific types of tumors. Ellipticine is also interesting as an anticancer drug as it has limited side effects and lacks of hematological toxicity. Various methods to study intercalating activity of this drug have been developed. However, to our best knowledge, capillary electrophoresis (CE) as a technique combining high separation resolution with various detection options has never been used for these purposes. In this study, a novel separation method based on CE with laser-induced fluorescence (CE-LIF) detection has been developed for the determination of ellipticine and for the monitoring of ellipticineDNA interaction. Sodium acetate (50 mM, pH 4.5) was used as a background electrolyte and LIF detection at ?ex = 488 nm. The limit of detection for ellipticine was determined to be 5 x 10(-8) M. A total of 20% d
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Electrophoresis
ISSN
0173-0835
e-ISSN
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Svazek periodika
33
Číslo periodika v rámci svazku
11
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
5
Strana od-do
1545-1549
Kód UT WoS článku
000305753600008
EID výsledku v databázi Scopus
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