Exploring the Potential of Fungal Arylacetonitrilases in Mandelic Acid Synthesis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10296584" target="_blank" >RIV/00216208:11310/15:10296584 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/15:00444847

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s12033-015-9840-y" target="_blank" >http://dx.doi.org/10.1007/s12033-015-9840-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12033-015-9840-y" target="_blank" >10.1007/s12033-015-9840-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Exploring the Potential of Fungal Arylacetonitrilases in Mandelic Acid Synthesis

Popis výsledku v původním jazyce

The application of arylacetonitrilases from filamentous fungi to the hydrolysis of high concentrations of (R, S)-mandelonitrile (100-500 mM) was demonstrated for the first time. Escherichia coli strains expressing the corresponding genes were used as whole-cell catalysts. Nitrilases from Aspergillus niger, Neurospora crassa, Nectria haematococca, and Arthroderma benhamiae (enzymes NitAn, NitNc, NitNh, and NitAb, respectively) exhibited different degrees of enantio-and chemoselectivity (amide formation).Their enantio-and chemoselectivity was increased by increasing pH (from 8 to 9-10) and adding 4-10 % (v/v) toluene as the cosolvent. NitAn and NitNc were able to convert an up to 500 mM substrate in batch mode. NitAn formed a very low amount of the by-product, amide (<1% of the total product). This enzyme produced up to >70 g/L of (R)-mandelic acid (e.e. 94.5-95.6 %) in batch or fed-batch mode. Its volumetric productivities were the highest in batch mode [571 +/- 32 g/(L d)] and its cat

Název v anglickém jazyce

Exploring the Potential of Fungal Arylacetonitrilases in Mandelic Acid Synthesis

Popis výsledku anglicky

The application of arylacetonitrilases from filamentous fungi to the hydrolysis of high concentrations of (R, S)-mandelonitrile (100-500 mM) was demonstrated for the first time. Escherichia coli strains expressing the corresponding genes were used as whole-cell catalysts. Nitrilases from Aspergillus niger, Neurospora crassa, Nectria haematococca, and Arthroderma benhamiae (enzymes NitAn, NitNc, NitNh, and NitAb, respectively) exhibited different degrees of enantio-and chemoselectivity (amide formation).Their enantio-and chemoselectivity was increased by increasing pH (from 8 to 9-10) and adding 4-10 % (v/v) toluene as the cosolvent. NitAn and NitNc were able to convert an up to 500 mM substrate in batch mode. NitAn formed a very low amount of the by-product, amide (<1% of the total product). This enzyme produced up to >70 g/L of (R)-mandelic acid (e.e. 94.5-95.6 %) in batch or fed-batch mode. Its volumetric productivities were the highest in batch mode [571 +/- 32 g/(L d)] and its cat

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP504%2F11%2F0394" target="_blank" >GAP504/11/0394: Mezidruhová analýza rekombinantních nitrilas exprimovaných z hypotetických sekvencí, jejich význam a potenciální aplikace v životním prostředí</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

ISSN

1073-6085

e-ISSN

—

Svazek periodika

57

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

466-474

Kód UT WoS článku

000353147700009

EID výsledku v databázi Scopus

2-s2.0-84928310446