Chromatographic Characterization of a New Cationic beta-CD Based Stationary Phase Prepared by Dynamic Coating

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10323993" target="_blank" >RIV/00216208:11310/16:10323993 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10337-016-3050-z" target="_blank" >http://dx.doi.org/10.1007/s10337-016-3050-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10337-016-3050-z" target="_blank" >10.1007/s10337-016-3050-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chromatographic Characterization of a New Cationic beta-CD Based Stationary Phase Prepared by Dynamic Coating

Popis výsledku v původním jazyce

Cyclodextrin-based stationary phases are frequently used for separation of various isomers. The main reason for applying new cyclodextrin (CD) derivatives for HPLC stationary phases is the improvement of separation efficiency. This paper introduces a new stationary phase prepared by dynamic coating of ion-exchanger with a newly synthesized cationic derivative of beta-cyclodextrin. This mono-substituted derivative of beta-cyclodextrin contains two tetraalkylammonium groups in its side chain. Diverse sets of analytes were tested to reveal the separation potential of the derivatized stationary phase. This dynamically coated stationary phase exhibited mostly better selectivity and resolution than a commercial chemically bonded beta-cyclodextrin stationary phase. Mixtures of analytes were baseline resolved in shorter analysis time. Furthermore, interactions participating in the retention mechanism were identified using a linear free energy relationship approach and ionic interaction study. Lower hydrophobicity and higher ability to interact as hydrogen bond donor affect retention on the coated stationary phase. Even though the ionic interaction study confirmed that the surface of the ion-exchanger was modified by dynamic coating, the ionic interactions still contributed to the retention mechanism on the coated stationary phase.

Název v anglickém jazyce

Chromatographic Characterization of a New Cationic beta-CD Based Stationary Phase Prepared by Dynamic Coating

Popis výsledku anglicky

Cyclodextrin-based stationary phases are frequently used for separation of various isomers. The main reason for applying new cyclodextrin (CD) derivatives for HPLC stationary phases is the improvement of separation efficiency. This paper introduces a new stationary phase prepared by dynamic coating of ion-exchanger with a newly synthesized cationic derivative of beta-cyclodextrin. This mono-substituted derivative of beta-cyclodextrin contains two tetraalkylammonium groups in its side chain. Diverse sets of analytes were tested to reveal the separation potential of the derivatized stationary phase. This dynamically coated stationary phase exhibited mostly better selectivity and resolution than a commercial chemically bonded beta-cyclodextrin stationary phase. Mixtures of analytes were baseline resolved in shorter analysis time. Furthermore, interactions participating in the retention mechanism were identified using a linear free energy relationship approach and ionic interaction study. Lower hydrophobicity and higher ability to interact as hydrogen bond donor affect retention on the coated stationary phase. Even though the ionic interaction study confirmed that the surface of the ion-exchanger was modified by dynamic coating, the ionic interactions still contributed to the retention mechanism on the coated stationary phase.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chromatographia

ISSN

0009-5893

e-ISSN

—

Svazek periodika

79

Číslo periodika v rámci svazku

9-10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

529-536

Kód UT WoS článku

000376125000001

EID výsledku v databázi Scopus

2-s2.0-84962689049