Impact of the central atom on human genomic DNA and human serum albumin binding properties in analogous Zn(II) and Cd(II) complexes with mefenamic acid

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10404374" target="_blank" >RIV/00216208:11310/19:10404374 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=hNBBR.b_yM" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=hNBBR.b_yM</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molstruc.2019.03.078" target="_blank" >10.1016/j.molstruc.2019.03.078</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Impact of the central atom on human genomic DNA and human serum albumin binding properties in analogous Zn(II) and Cd(II) complexes with mefenamic acid

Popis výsledku v původním jazyce

Two novel complexes of Zn(II) and Cd(II) with Hmef (mefenamic acid) and bapen (N,N&apos;-bis(3-aminopropyl)ethylenediamine) ligand were synthesized and characterized by infrared spectroscopy, elemental, thermal and single-crystal X-ray structure analysis. Both complexes form molecular structure composed of analogous neutral complex molecules [M(bapen)(mef)(2)], which differ in the central atom and a solvate molecule - Zn(II) in 1 [Zn(bapen)(mef)(2)] is replaced by Cd(II) in the compound 2 [Cd(bapen)(mef)(2)] center dot MeOH. The interactions of both complexes with human genomic DNA isolated from tissues of pelvis uteri affected by precancerosis and cancer were studied by competitive binding studies with ethidium bromide (EB) and UV-VIS titration. The obtained values of Stern-Volmer constants K-SV and the values of binding constants K-b follow the same trend, indicating different affinity of the studied complexes towards the DNA samples; stronger binding was observed in case of DNA samples from tissue with more severe damage. In addition, binding of the prepared complexes with human serum albumin (HSA) was investigated and the observed K-HSA values are within optimal range for transport in bloodstream.

Název v anglickém jazyce

Impact of the central atom on human genomic DNA and human serum albumin binding properties in analogous Zn(II) and Cd(II) complexes with mefenamic acid

Popis výsledku anglicky

Two novel complexes of Zn(II) and Cd(II) with Hmef (mefenamic acid) and bapen (N,N&apos;-bis(3-aminopropyl)ethylenediamine) ligand were synthesized and characterized by infrared spectroscopy, elemental, thermal and single-crystal X-ray structure analysis. Both complexes form molecular structure composed of analogous neutral complex molecules [M(bapen)(mef)(2)], which differ in the central atom and a solvate molecule - Zn(II) in 1 [Zn(bapen)(mef)(2)] is replaced by Cd(II) in the compound 2 [Cd(bapen)(mef)(2)] center dot MeOH. The interactions of both complexes with human genomic DNA isolated from tissues of pelvis uteri affected by precancerosis and cancer were studied by competitive binding studies with ethidium bromide (EB) and UV-VIS titration. The obtained values of Stern-Volmer constants K-SV and the values of binding constants K-b follow the same trend, indicating different affinity of the studied complexes towards the DNA samples; stronger binding was observed in case of DNA samples from tissue with more severe damage. In addition, binding of the prepared complexes with human serum albumin (HSA) was investigated and the observed K-HSA values are within optimal range for transport in bloodstream.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10402 - Inorganic and nuclear chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Molecular Structure

ISSN

0022-2860

e-ISSN

—

Svazek periodika

1188

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

42-50

Kód UT WoS článku

000464988000006

EID výsledku v databázi Scopus

2-s2.0-85063634266