A UV-Vis method for investigation of gallium(III) complexation kinetics with NOTA and TRAP chelators: advantages, limitations and comparison with radiolabelling
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F24%3A10490649" target="_blank" >RIV/00216208:11310/24:10490649 - isvavai.cz</a>
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Hqst-kVpc_" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Hqst-kVpc_</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/d4dt02383h" target="_blank" >10.1039/d4dt02383h</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
A UV-Vis method for investigation of gallium(III) complexation kinetics with NOTA and TRAP chelators: advantages, limitations and comparison with radiolabelling
Popis výsledku v původním jazyce
An easy and cheap method for measurement of Ga(III) complexation kinetics was developed. The method is based on UV-Vis quantification of non-complexed chelators after the addition of Cu(II) ions at individual time points. The method was evaluated using established ligands, H(3)nota and H(6)notP(Pr), and was utilized to study the kinetics of Ga-III complexation with four new symmetric derivatives of 1,4,7-triazacyclononane bearing methylphosphonate/phosphinate pendant arms - TRAP ligands. Chelators bearing ethoxy groups (H(3)L1) or 2,2,2-trifluoroethyl groups (H(3)L2) on the phosphorus atoms showed fast formation (t(99%) = 21 and 10 min, respectively, at pH 2.0) and efficient radiolabelling which were comparable to the previously reported chelators bearing the 2-carboxyethyl group (H(6)notP(Pr)). Chelators bearing (N,N-dibenzyl-amino)methyl (H(3)L3) and aminomethyl (H(3)L4) substituents showed a significantly slower complexation (t(99%) = 4.4 and 3.6 h, respectively, at pH 2.0) and inefficient radiolabelling, mainly at room temperature or low pH. This was caused by protonation of the amino groups of the pendant arms leading to coulombic repulsion between the Ga(III) ion and the positively charged protonated amines. The trends in complexation rates determined by the UV-Vis method correlated well with the results of the Ga-68 radiolabelling study.
Název v anglickém jazyce
A UV-Vis method for investigation of gallium(III) complexation kinetics with NOTA and TRAP chelators: advantages, limitations and comparison with radiolabelling
Popis výsledku anglicky
An easy and cheap method for measurement of Ga(III) complexation kinetics was developed. The method is based on UV-Vis quantification of non-complexed chelators after the addition of Cu(II) ions at individual time points. The method was evaluated using established ligands, H(3)nota and H(6)notP(Pr), and was utilized to study the kinetics of Ga-III complexation with four new symmetric derivatives of 1,4,7-triazacyclononane bearing methylphosphonate/phosphinate pendant arms - TRAP ligands. Chelators bearing ethoxy groups (H(3)L1) or 2,2,2-trifluoroethyl groups (H(3)L2) on the phosphorus atoms showed fast formation (t(99%) = 21 and 10 min, respectively, at pH 2.0) and efficient radiolabelling which were comparable to the previously reported chelators bearing the 2-carboxyethyl group (H(6)notP(Pr)). Chelators bearing (N,N-dibenzyl-amino)methyl (H(3)L3) and aminomethyl (H(3)L4) substituents showed a significantly slower complexation (t(99%) = 4.4 and 3.6 h, respectively, at pH 2.0) and inefficient radiolabelling, mainly at room temperature or low pH. This was caused by protonation of the amino groups of the pendant arms leading to coulombic repulsion between the Ga(III) ion and the positively charged protonated amines. The trends in complexation rates determined by the UV-Vis method correlated well with the results of the Ga-68 radiolabelling study.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10402 - Inorganic and nuclear chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/TH78020003" target="_blank" >TH78020003: Zobrazovací a inhibiční sondy pro diagnostiku a léčení Alzheimerovy choroby</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2024
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Dalton Transactions
ISSN
1477-9226
e-ISSN
1477-9234
Svazek periodika
53
Číslo periodika v rámci svazku
43
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
11
Strana od-do
17554-17564
Kód UT WoS článku
001337005800001
EID výsledku v databázi Scopus
2-s2.0-85206602870