Comparison ot the syphilis and yaws spirochete genomes: comparative genome sequencing of T. pallidum ssp. pallidum Samoa D.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F06%3A00016083" target="_blank" >RIV/00216224:14110/06:00016083 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Comparison ot the syphilis and yaws spirochete genomes: comparative genome sequencing of T. pallidum ssp. pallidum Samoa D.
Popis výsledku v původním jazyce
Spirochetal genus Treponema includes several closely related pathogenic spirochetes. Treponema pallidum ssp. pallidum (TPA) is the causative agent of syphilis, T. pallidum ssp. pertenue (TPE) causes yaws. Although these two subspecies are microscopicallyand serologically indistinguishable, they cause different diseases. Little is known about genome differences between TPA and TPE genomes. The aim of this work was to employ dideoxyterminator sequencing for sequence differences discovery between Nichols(TPA) and Samoa D (TPE) genomes. All heterologous regions were successfully subjected to DDT sequencing and consensus sequences were obtained using three different approaches: 1) direct PCR product sequencing, 2) primer walking and 3) small insert library sequencing. Identified sequence differences will be used together with CGS data to compile the complete genome sequence of Samoa D strain.
Název v anglickém jazyce
Comparison ot the syphilis and yaws spirochete genomes: comparative genome sequencing of T. pallidum ssp. pallidum Samoa D.
Popis výsledku anglicky
Spirochetal genus Treponema includes several closely related pathogenic spirochetes. Treponema pallidum ssp. pallidum (TPA) is the causative agent of syphilis, T. pallidum ssp. pertenue (TPE) causes yaws. Although these two subspecies are microscopicallyand serologically indistinguishable, they cause different diseases. Little is known about genome differences between TPA and TPE genomes. The aim of this work was to employ dideoxyterminator sequencing for sequence differences discovery between Nichols(TPA) and Samoa D (TPE) genomes. All heterologous regions were successfully subjected to DDT sequencing and consensus sequences were obtained using three different approaches: 1) direct PCR product sequencing, 2) primer walking and 3) small insert library sequencing. Identified sequence differences will be used together with CGS data to compile the complete genome sequence of Samoa D strain.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2006
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů