HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14160%2F24%3A00135742" target="_blank" >RIV/00216224:14160/24:00135742 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2297-8739/11/2/45" target="_blank" >https://www.mdpi.com/2297-8739/11/2/45</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/separations11020045" target="_blank" >10.3390/separations11020045</a>

Jazyk výsledku

angličtina

Název v původním jazyce

HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements

Popis výsledku v původním jazyce

This article highlights the fundamental aspects of hydrophilic interaction liquid chromatography (HILIC) on poly-hydroxyl stationary phases to analyze non-derivatized mono- and disaccharides, including commonly consumed carbohydrates like glucose, fructose, sucrose, and lactose. The evaporative light-scattering detector (ELSD) is utilized as an alternative to an MS detector, and the separation system's selectivity allows the separation of anomers of monosaccharides. The study also includes a rapid method for determining isomaltulose (Palatinose), which was validated and applied to food supplement samples available in the Czech market, even those with high protein content. Additionally, isomaltulose was separated from sucrose in just 13 min.

Název v anglickém jazyce

HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements

Popis výsledku anglicky

This article highlights the fundamental aspects of hydrophilic interaction liquid chromatography (HILIC) on poly-hydroxyl stationary phases to analyze non-derivatized mono- and disaccharides, including commonly consumed carbohydrates like glucose, fructose, sucrose, and lactose. The evaporative light-scattering detector (ELSD) is utilized as an alternative to an MS detector, and the separation system's selectivity allows the separation of anomers of monosaccharides. The study also includes a rapid method for determining isomaltulose (Palatinose), which was validated and applied to food supplement samples available in the Czech market, even those with high protein content. Additionally, isomaltulose was separated from sucrose in just 13 min.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fluid - Particle Separations Journal

ISSN

1541-9290

e-ISSN

2297-8739

Svazek periodika

11

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

16

Strana od-do

1-16

Kód UT WoS článku

001172378500001

EID výsledku v databázi Scopus

2-s2.0-85185979266